阴离子表面活性剂存在下氯化血红素催化性能的研究

研究了十二烷基苯磺酸钠 (SDBS)存在下氯化血红素 (Hemin)类似过氧化物酶的新型催化活性。在 pH 8 0～ 8 9氨性缓冲介质中 ,Hemin催化溶解氧氧化溴邻苯三酚红的褪色反应。在SDBS和 30 %乙醇的增效作用下 ,催化体系服从Michaelis Menten方程。用Lineweaver Burk作图法求得米氏常数、催化常数和专一性常数分别为 3 7× 10 - 5mol/L ,0 6 4s- 1和 1 7× 10 4 L·mol- 1·s- 1。对机理进行了讨论。利用该催化体系 ,用反应平衡法和动力学法均可分光光度定量Hemin ,线性范围为 2 0× 10 - 8～ 6 0× 10 - 7mol/L ,检测限为 2 5× 10 - 9mol/L

Study on Catalytic Property of Hemin in the Presence of Anion Surfactant

A novel catalytic property of hemin is described.In ammonium buffer medium of pH 8.0～8.9,hemin has an ability to catalyze the decoloration of bromopyro-gallol red.Oxygen dissolved in solution plays a role as an oxidant in the reaction.With sodium dodecylbenzenesulfonate and ethanol as effect-enhancing agents,the catalytic system obeys the Michaelis-Menten equation of enzymatic catalysis.The Michaelis constant,catalytic constant and specificity constant of the system are estimated by Lineweaver-Burk plot to be 3.7×10-5?mol/L,0.64?s-1 and 1.7×104?L*mol-1*s-1,respectively.The mechanism of the hemin oxidase-like catalytic system is discussed.Based on this,hemin can be spectrophotometrically determined by equilibrium and kinetic procedures with a linear range and 3σ limit of detection being 2.0×10-8～6.0×10-7 and 5.0×10-9?mol/L,respectively.