| 山核桃外果皮黄酮提取条件的研究 |
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| 引用本文： | 查甫本,陈向明.山核桃外果皮黄酮提取条件的研究[J].合肥学院学报(自然科学版),2009,19(3):68-72. |
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| 作者姓名： | 查甫本 陈向明 |
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| 作者单位： | 1. 合肥工业大学,生物与食品工程学院,合肥,230009
2. 合肥学院,生物与环境工程系,合肥,230022 |
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| 基金项目： | 安徽省高等学校省级自然科学基金项目 |
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| 摘 要： | 采用兮光光度法，以芦丁为对照品，乙醇回流萃取山核桃外果支中总黄酮．在革取过程中，通过单因素试验分析了乙醇浓度、苹取温度、萃取时间及料液比等4个主要因素对萃取率的影响．通过正交试验，得到最佳萃取工艺条件为：乙醇浓度为70％，萃取温度60℃，萃取时间为6h，料液比1：20．试验方法简便，结果可靠．
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| 关 键 词： | 分光光度法 山核桃外果支 总黄酮 正交试验 |
Studies on Extraction Technique of Total Flavonoids from C.cathayensis Epicarp |
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| ZHA Fu-ben,CHEN Xiang-ming.Studies on Extraction Technique of Total Flavonoids from C.cathayensis Epicarp[J].Journal of Hefei University :Natural Sciences,2009,19(3):68-72. |
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| Authors: | ZHA Fu-ben CHEN Xiang-ming |
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| Institution: | 1.School of Biotechnology and Food Engineering;Hefei University of Technology;Hefei 230009;2.Department of Biological and Environmental Engineering;Hefei University;Hefei 230022;China |
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| Abstract: | Spectrometric method was employed to measure the content of total flavonoids in C. cathayensis epicarp using ethanol as solvent, rutin as control sample. During the extracting process, four main factors including concentration of extracting solution, temperature, time and solid - liquid ratio are studied by using single factor analysis method. The optimal conditions are obtained by orthognnal analysis, which are 70% of methanol concentration, 6h of refluxing duration, 60℃ of refluxing temperature and 1 ： 20 of solid-liquid ratio. The method is simple and convenient . |
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| Keywords: | spectrometric C cathayensis epicarp total flavonoids orthogonal test |
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