| 用正交设计优化甜菜RAPD反应体系 |
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| 引用本文： | 腊萍,罗淑萍.用正交设计优化甜菜RAPD反应体系[J].伊犁师范学院学报(自然科学版),2009(3):24-30. |
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| 作者姓名： | 腊萍 罗淑萍 |
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| 作者单位： | 1. 伊犁师范学院,化学与生物科学学院,新疆,伊宁,835000
2. 新疆农业大学,农学院,新疆,乌鲁木齐,830052 |
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| 摘 要： | 采用正交设计对甜菜的RAPD反应体系进行优化．应用L25（5^6）正交表，研究了Taq、Mg^2＋、随机引物、dNTPs和DNA模板5种RAPD反应组分浓度变化对扩增结果的影响，并进行量化分析，试验结果表明用这种方法建立的甜菜RAPD-PCR优化反应体系为：25μL反应体系中含1×Buffer、1．0mmol／L Mg^2＋、1U TaqDNA聚合酶、0．25mmol／L dNTPs、0．4μmol／L随机引物及25ng DNA模板．
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| 关 键 词： | 甜菜 RAPD 反应体系 正交设计 |
Optimization of the RAPD Reaction System of Sugar Beet by Orthogonal Design |
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| LA Ping,LUO Shu-ping.Optimization of the RAPD Reaction System of Sugar Beet by Orthogonal Design[J].Journal of Ili Normal University,2009(3):24-30. |
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| Authors: | LA Ping LUO Shu-ping |
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| Institution: | LA Ping, LUO Shu-ping (1. Chemistry ＆ Biology College, Yili Normal University, Yining, Xinjiang 835000; 2. Agricultural Science College, Xinjiang Agricultural University, Urumqi, Xinjiang 830052) |
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| Abstract: | The L25 （56） orthogonal diagram was applied to study the effects of different concentrations of Taqpolymerase, Mg^2＋, dNTPs, primer and DNA on sugar beet. The results showed that the orthogonal design could be used to optimize the RAPD-PCR system. A suitable RAPD reaction system for Sugar beet was developed, 1 ×Taq polymerase reaction buffer, 1.0mmol/L Mg^2＋, 1U Taq DNA polymerase, 0.25mmol/L dNTPs, 0.4μmol/L primer and 25ng genomic DNA template with total 25μL reaction solution.. |
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| Keywords: | RAPD |
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