透明质酸产生菌的诱变育种及发酵条件

本文研究了透明质酸（ＨＡ）产生菌的诱变育种及摇瓶发酵条件。以兽疫链球菌（Ｓｔｒｅｐｔｏｃｏｃｃｕｓｚｏｅｐｉｄｅｍｉｃｃｕｓ）ＮＣＴ６１８０为出发菌种，在普通加血培养基上研究了ＨＡ的产生能力，用正交法研究了野生菌的最佳培养条件，通过单菌落分离得到野生高产菌株。再通过紫外和ＮＴＧ诱变得到溶血性较弱的菌株，部分菌株比出发菌株产量提高一倍以上。在摇瓶条件下，ＰＨ值的控制及葡萄糖的加入均有利于ＨＡ的产生。

Studies on mutation breeding and fermentational condition by Streptococcus zooepidemiccus

rimary researches on the mutation breeding of Streptococcus zooepidemiccusand its shaker-flask fermentational conditions were carried out. Wild Streptococcus zOOepidemiccus NCTC 6180 was adopted, its ability of HA-producing wade examined by commonblood medium, and its optimum cultivation conditions were studied by orthogonal method.From the fermentational results of single colony, a high HA-producing strain was acquired, further more studies on its UV-mutation and NTG-mutation were conducted, and meanwhile some strains low-homoclasis were obtained; a number of those colonys canproduce 100% HA more than the original strain. The controlling of PH and the adding of glucose under the condition of shaker-flask are beneficial to the production of HA.