Separation of DNA fragments by cross-linked polymer coating capillary electrophoresis with non-gel sieving matrix |
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Authors: | Chen Hong Song Liguo Cheng Jieke |
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Institution: | (1) Department of Chemistry, Wuhan University, 430072 Wuhan, China |
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Abstract: | A new type of flush/fill equipment driven by high-pressure (1MPa) nitrogen gas for filling the capillary is demonstrated. High-viscous and microlitre grade volume solution can be filled into a capillary by means of this equipment. The capillary coated by cross-linked polyacrylamide and filled with “interpenetrating polymer networks” non-gels sieving matrix (0.8% hydroxyethyl cellulose, 10% sucrose in 1×TBE buffer) was applied for separating DNA fragments. All 11 fragments of Ø174/Hae III DNA were successfully separated. The man-made circle plasmid from E. coli were separated and detected. |
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Keywords: | capillary electrophoresis cross-linked polyacrylamide coating non-gels sieving matrix DNA fragments circle plasmid DNA |
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