Effect of iron deficiency on heterocyst differentiation and physiology of the filamentous cyanobacterium<Emphasis Type="Italic">Anabaena</Emphasis> sp. PCC 7120 |
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Authors: | Xu?Wen-liang Email author" target="_blank">Liu?Yong-dingEmail author Zhang?Cheng-cai |
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Institution: | (1) Institute of Hydrobiology, Chinese Academy of Sciences, 430072 Wuhan, Hubei, China;(2) Laboratoire de Chimie Bacterienne (UPR9043), CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille cedex 20, France;(3) College of Life Sciences and Technology, Huazhong Agricultural University, 430070 Wuhan, Hubei, China |
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Abstract: | The effect of iron deficiency on heterocyst differentiation and some physiological properties of the filamentous cyanobacteriumAnabaena sp. PCC 7120 was investigated. Under moderate iron limitation conditions, achieved by addition of iron chelator 2, 2′-Dipyridyl
(<80 μmol/L) led to delayed heterocyst differentiation, no heterocyst differentiation was observed under severe iron limitation
conditions, when the concentration of 2,2′-Dipyridyl in the medium was more than 100 μmol/L. it seemed that there are certain
iron-regulated genes or operons whose function is to control heterocyst development. In addition, iron deficiency impaired
the growth. Low iron cells had a decrease in the quantities of pigment content (chlorophyll and phycocyanin content), the
whole cellin vivo absorbance spectra confirmed the decrease, the protein electrophoretic profiles revealed that iron-deficient cells had less
protein bands, with the increase of 2,2′-Dipyridyl, the protein bands was more and more less. And differently, iron deficiency
also caused an increase of ROS (Reactive Oxygen Species) and SOD activity, it suggests that iron deficiency led to oxidative
stress, which generally occured under high-iron conditions.
Foundation item: Supported by the National Natural Sciences Foundation of China (30070154), the Frontier Science Projects Programme of the
Institute of Hydrobiology, the Chinese Academy of Sciences (220316), State Key Project on Cyanobacterial Bloom Control in
Lake Danchi (K9905-35-01)
Biography: Xu Wenllang (1974-), male, Ph. D. research direction: molecular genetics of eyanobacteria. |
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Keywords: | Anabaena sp PCC 7120 iron deficiency heterocyst development protein SDS-PAGE oxicative stress |
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