Regenerating plants from cryopreserved adventitious buds of haploids in rice |
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Authors: | Zhang Zhihong Hu Zhangli |
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Institution: | (1) College of Life Sciences, Wuhan University, 430072 Wuhan, China |
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Abstract: | A large number of adventitious buds were induced fromin vitro cultured young inflorescences of haploids of rice. Having been subcultured on solidified subculture media at 26°C for 7 days,
the adventitious buds were loaded into 1.8 mL plastic cryotubes with cryoprotectant and kept on ice for 45–60 min. After cooled
at a rate of 1.0°C/min down to −40°C, the samples, were kept in liquid nitrogen. The adventitious buds which have been cryopreserved
for about 30 days were thawed rapidly in 38–40°C water and then plated on solidified MS medium containing 3% sucrose, 0.5
mg/L NAA and 2.0 mg/L kinetin. After plated, 23%–32% of adventitious buds resumed growth and 15%–22% regenerated plantlets.
The results of this work indicated that the adventitious buds derived fromin vitro cultured young inflorescences is a critical factor for the success and subculturing adventitious buds on MS medium containing
3% sucrose and 4% sorbitol or 20% potato extract is essential to the procedure. The effective cryoprotectant is 10% DMSO (dimethyl
sulfoximide)+0.5 mol/L sorbitol.
Supported by the Natural Science Foundation of Hubei Province
Zhang Zhihong: born in 1963, Lecturer |
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Keywords: | Oriza sativa haploid adventitious bud cryopreservation |
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