| The Expression of vasa Gene during Zebrafish （Danio rerio） Oogenesis |
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| 作者姓名： | XIANGFang ZHENYan ZHENGWen-xuan DENGFeng-jiao WANGXiao-kai ZHANGXi-yuan |
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| 作者单位： | [1]KeyLaboratoryofMOEforDevelopmentalBiology,CollegeofLifeSciences,WuhanUniversity,Wuhan430072,Hubei,China [2]WuhanMunicipalFourthHospital,Wuhan430033,Hubei,China |
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| 基金项目： | theNationalNaturalScienceFoundationofChina (30 370 744,30 1 50 0 0 5) |
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| 摘 要： | Vasa gene expression pattern during oogenesis of zebrafish was examined using in situ hybridization and fluorescent quantitative RT-PCR During zebrafish oogensis, vasa mRNA is expressed strongly and uniformly distributed in the cytoplasm in stage Ⅱ oocytes, followed by a distribution among vacuome in stage Ⅲ. Later in stage Ⅳ and Ⅴ, vasa mRNA is enriched at the cortex and finally localized at the cortex. The fluorescent quantitative RT-PCR shows that the quantity of vasa mRNA decreases from stage Ⅱ to stage Ⅲ, but remains relatively invariable from stage Ⅲ to stage Ⅴ. The observed differences in vasa mRNA expression in the different stages of zebrafish oogenesis suggest that vasa gene plays an important role during oogenesis.
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| 关 键 词： | 基因表达 斑马鱼 鲐 卵子发生 杂交 mRNA RT-PCR 荧光定量分析 |
The Expression of vasa Gene during Zebrafish (Danio rerio) Oogenesis |
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| XIANGFang ZHENYan ZHENGWen-xuan DENGFeng-jiao WANGXiao-kai ZHANGXi-yuan.The Expression of vasa Gene during Zebrafish (Danio rerio) Oogenesis[J].Wuhan University Journal of Natural Sciences,2004,9(6):979-982. |
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| Abstract: | |
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| Keywords: | vasa zebrafish fluorescent quantitative RT-PCR oogenesis |
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