An interspecies conserved antigen ofPlasmodium falciparum containing clusters of asparagine residues |
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| Authors: | Ma Donghui Li Xiaojie Ma Huiwen Fred A S Kironde |
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| Institution: | (1) College of Life Sciences, Wuhan University, 430072 Wuhan, China;(2) International Center for Genetic Engineering and Biotechnology, Shaheed Jeet Singh Marg, 110067 New Delhi, India |
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| Abstract: | An interspecies conservedPlasmodium asparagine rich antigen, designated as ARK26, was isolated by immunoscreeningP. falciparum genomic DNA expression library with mouse convalescent anti-P. yeolii serum. Partial DNA sequence analysis reveals that ARK26 contains clusters of asparagines and no randomly repeated amino acid
sequence motifs are observed. A 65×103 GST fusion protein is expressed by recombinant plasmid PGEX-5X-1 (ARK26) inE. coli C strain ABLE-K. Computer programs predict that two asparagine rich regions are among the possible antigenic epitopes of
p37 encoded by ARK26. Interestingly, the sequence of ARK26 displays significant similarity to yeast and several other species’
mitochondrial genes, and its possible function is discussed.
Supported by a fellowship offered by International Center for Genetic Engineering & Biotechnology(ICGEB)
Ma Donghui: born in 1969, Graduate student |
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| Keywords: | Plasmodium falciparum asparagine-rich proteins interspecies conserved antigens |
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