| Heterologous Expression of Mycobacterium tuberculosis AgSSB in Pichia pastoris |
| |
| 摘 要： |
|
| 关 键 词： | 毕赤酵母 分泌器官 分歧杆菌属 肺结核 Ag85B |
| 收稿时间： | 30 June 2004 |
Heterologous expression ofMycobacterium tuberculosis Ag85B inPichia pastoris |
| |
| Authors: | Liu Yan Peng Jian-hong Ma Li-xin Ju Wei Qu Xue-ju Liu Jun-yan |
| |
| Institution: | (1) Department of Immunology, School of Medicine, Wuhan University, 430071 Wuhan, Hubei, China;(2) Laboratory of Molecular Microbiology & Gene Engineering, Faculty of Life Science, Hubei University, 430064 Wuhan, Hubei, China;(3) Institute of Allergy and Immune-Related Disease, School of Medicine, Wuhan University, 430071 Wuhan, Hubei, China |
| |
| Abstract: | The DNA fragment encoding matureMycobacterium tuberculosis major secretory protein Ag85B was inserted into thePichia pastoris secretory expression vector pHBM905A, under the control of theAOX1 promoter. The recombinant plasmid pHBM905A-85B linearized bySal I was introduced intoPichia pastoris strain GS115 by PEG1000 transformation method. After phenotype screening and PCR identification, the resulting GS115-pHBM905A-85B
strain was cultivated and induced with methanol. The recombinant Ag85B protein in secreted form was attained with molecular
weight of 35×103 approximately detected by SDS-PAGE and Western blot. ELISA experiment proved that the protein had good antigen specificity.
Secretory expression of recombinantM. tuberculosis Ag85B inP. pastoris will open a door to mass production of the protein in heterologous host and allow ready evaluation of its immunological function.
Foundation item: Supported by the Key Scientific and Technological Project of Wuhan(301121028)
Biography: LIU Yan(1971-), female, Ph. D candidate, research direction: vaccine against tuberculosis. |
| |
| Keywords: | Pichia pastoris secretory expression Mycobacterium tuberculosis Ag85B |
| 本文献已被 SpringerLink 等数据库收录！ |
|
