A Tumor Suppressor Gene fhit Prokaryotic Expression and Identification

The recomblnant expression vector pGEMD-fhit wMch contains full encoding region of fhit gene was constructed. The recomMnant was introduced into the BL21 (DE3) strain of E. coli and induced by 1 mmol/L IPTG to express a 29× 103 polypeptide of fhit fusion protein. And the 29× 103 protein was sensitive and specific in reaction with anti-fhit antibody in Western blot.