Organic solvents enhanced spectrofluorimetric method for determination of laccase activity |
| |
Authors: | Huang Zuyun Liu Zhihong Cai Ruxiu and Zeng Yun'e |
| |
Institution: | (1) Department of Chemistry, Wuhan University, 430072 Wuhan, China |
| |
Abstract: | A novel fluorimetric method for determination of laccase activity in organic solvents is proposed, based on the oxidation
ofo-phenylenediamine (1,2-diaminobenzene, OPDA) catalyzed by laccase yielding 2,3-diaminophenazine. The optimal conditions for
laccase in organic media areT=55°C, pH=6.5, 1.0×10−2mol/L OPDA, 1.25 mL ethanol, 1.25 mL 1,4-dioxane and 1.25 mL acetone. The linear range of the method proposed in ethanol,
1,4-dioxane and acetone media were 0.44–19.33, 0.11–20.85, 0.38–21.05 U with the detection limit of 0.088, 0.022, 0.076 U,
respectively. The proposed method has been applied to the analysis of laccase activity of real samples with more accurate
and sensitive than that of the previous method reported.
Supported by the National Natural Science Foundation of China and the Special Founds of State Education Committee for Doctorate
Research
Huang Zuyun: born in 1963, Ph.D |
| |
Keywords: | Organic solvent laccase activity fluorimetry o-phenylenediamine (OPDA) |
本文献已被 SpringerLink 等数据库收录! |
|