| 腈水合酶产生菌 Rhodococcus sp.HUST-1发酵条件的优化 |
| |
| 引用本文： | 朱婷婷,秦智,孙晓君,蒋忠平.腈水合酶产生菌 Rhodococcus sp.HUST-1发酵条件的优化[J].江西师范大学学报(自然科学版),2008,32(6). |
| |
| 作者姓名： | 朱婷婷 秦智 孙晓君 蒋忠平 |
| |
| 作者单位： | 1. 哈尔滨理工大学,化学与环境工程学院,黑龙江,哈尔滨,150040;新余高等专科学校太阳能科学与工程系,江西,新余,338000
2. 哈尔滨理工大学,化学与环境工程学院,黑龙江,哈尔滨,150040 |
| |
| 基金项目： | 黑龙江省自然科学基金(E2004-08);;黑龙江省普通高等学校青年学术骨干支持计划(1153G008);;哈尔滨市科技攻关项目(2004AA4CS049);;哈尔滨市青年科学研究基金(2004AFQXJ041);;新余高等专科学校重点校级课题(XJ0739)资助项目 |
| |
| 摘 要： | Rhodococcus sp.HUST-1在Co2+的诱导下产腈水合酶,催化丙烯腈水合生成丙烯酰胺.对菌株HUST-1的产酶条件进行优化.研究表明,发酵过程中培养基中的碳氮源、诱导剂浓度以及培养条件影响腈水合酶的高效表达.在葡萄糖20 g/L、酵母粉10 g/L、Co2+0.08 mmol/L、酪氨酸0.2 g/L、pH7.0、培养温度28℃时,HUST-1所产腈水合酶总酶活达1 012.7 U,比优化前提高了47.1%.
|
| 关 键 词： | 腈水合酶 发酵条件 丙烯腈 |
Optimization of Fermentation Conditions for Nitrile Hydratase Producing Microognism Rhodococcus sp. HUST-1 |
| |
| ZHU Ting-ting,QIN Zhi,SUN Xiao-jun,JIANG Zhong-ping.Optimization of Fermentation Conditions for Nitrile Hydratase Producing Microognism Rhodococcus sp. HUST-1[J].Journal of Jiangxi Normal University (Natural Sciences Edition),2008,32(6). |
| |
| Authors: | ZHU Ting-ting QIN Zhi SUN Xiao-jun JIANG Zhong-ping |
| |
| Institution: | 1.School of Chemistry and Enviroment Engineering;Harbin University of Science and Technology;Harbin 150040;China;2.Department of Solar Energy Science and Engineering;Xinyu College;Xinyu Jiangxi 338001;China |
| |
| Abstract: | Rhodococcus sp.HUST-1 produces nitrile hydratase induced by Co2+,as a catalyst which can synthesize acrylamide from hydration of acrylonitrile.Through optimizing fermentation conditions of the nitrile hydratase producing strain HUST-1,results showed that over-expression of nitrile hydratase was influenced by the concentration of carbon source,nitrogen source,inducer in culture medium and cultivated conditions.The optimal fermentation conditions were as the following: the concentration of glucose 20 g/L,yeas... |
| |
| Keywords: | nitrile hydratase fermentation conditions acrylonitrile |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
