| 紫外诱变原生质体选育高产L-乳酸菌株的研究 |
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| 引用本文： | 仲松,张庆庆,孙平平.紫外诱变原生质体选育高产L-乳酸菌株的研究[J].安徽工程科技学院学报,2011,26(1). |
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| 作者姓名： | 仲松 张庆庆 孙平平 |
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| 作者单位： | 安徽工程大学生物与化学工程学院,安徽,芜湖,241000 |
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| 基金项目： | 安徽省科技攻关计划基金资助项目，安徽省高校自然科学基金资助项目 |
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| 摘 要： | 以干酪乳杆菌ZZ-L为出发菌株,对其原生质体制备条件进行了优化.结果表明,用为0.4 mg/mL的溶菌酶溶液和0.7 mol/L NaCl溶液作为渗稳剂对菌龄为22 h的干酪乳杆菌ZZ-L酶解时间110 min,原生质体的形成率为89.5%,再生率为47.7%,达到最佳水平.并对原生质体进行了紫外诱变育种.通过平板和静置发酵实验,筛选得到8株突变株产量比出发菌株高的突变株,其中菌株ZZ-06的L-乳酸产量达78.6 g/L,比出发菌种提高了20.7%,突变株ZZ06经12次传代,其遗传性状稳定.
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| 关 键 词： | 干酪乳杆菌 L-乳酸 原生质体 紫外诱变 |
Breeding of higher L-lactic acid mutants by protoplast mutagenesis |
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| ZHONG Song,ZHANG Qing-qing,SUN Ping-ping.Breeding of higher L-lactic acid mutants by protoplast mutagenesis[J].Journal of Anhui University of Technology and Science,2011,26(1). |
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| Authors: | ZHONG Song ZHANG Qing-qing SUN Ping-ping |
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| Institution: | ZHONG Song,ZHANG Qing-qing,SUN Ping-ping(Coll.of Bioch.Engn.,Anhui Polytechnic University,Wuhu 241000,China) |
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| Abstract: | Using Lactobacillus.casei ZZ-L,the optimal conditions of the protoplast preparation were studied in this experiment.The result showed that 22h cultured Lactobacillus.casei ZZ-L as material,0.7mol/L NaCl and 0.4mg/ml lysozyme were the optimum conditions of the protoplast preparation in 110mins.In the optimum conditions,preparation rate was 89.5% while the regeneration rate was 47.7%.The protoplast was used as the material in the UV-mutagenesis method for inducement.Then through plate screening and flask scre... |
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| Keywords: | lactobacillus casei L-lactic acid protoplast ultraviolet irritation |
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