| 人源抗菌肽LL-37原核表达载体的构建及表达 |
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| 引用本文： | 顾银霞,周学章,宋振威,李敏,王玉炯.人源抗菌肽LL-37原核表达载体的构建及表达[J].宁夏大学学报(自然科学版),2008,29(4). |
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| 作者姓名： | 顾银霞 周学章 宋振威 李敏 王玉炯 |
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| 作者单位： | 宁夏大学,西部特色生物资源保护与利用教育部重点实验室,宁夏,银川,750021 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 为了使人源抗菌肽LL-37在原核表达载体中获得高效表达,在不改变LL-37氨基酸的基础上,根据大肠杆菌偏爱密码子表,替换LL-37部分密码子,设计一段新的LL-37序列.采用DNA Work设计4条互补引物,利用SOE-PCR技术扩增完整的LL-37目的片段,以pET-PPPI为载体,构建原核表达载体pET-PPPIL,并制备表达菌BL21(DE3)(pET-PPPIL),该菌经诱导高效表达LL-37融合蛋白,为后续纯化LL-37奠定了基础.
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| 关 键 词： | 聚合酶链式反应 人源抗菌肽LL-37 蛋白表达 |
Construction of Human Antimicrobial Peptide LL-37 Prokaryotic Expression Vector and Its Expression |
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| Gu Yinxia,Zhou Xuezhang,Song Zhenwei,Li Min,Wang Yujiong.Construction of Human Antimicrobial Peptide LL-37 Prokaryotic Expression Vector and Its Expression[J].Journal of Ningxia University(Natural Science Edition),2008,29(4). |
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| Authors: | Gu Yinxia Zhou Xuezhang Song Zhenwei Li Min Wang Yujiong |
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| Abstract: | To make highly expressed human antimicrobial peptide LL-37 in prokaryotic expression system,the part of codon of LL-37 was replaced and a new LL-37 series was designed in this work.Four complementary primers were designed by DNA Work,the integrity of the LL-37 fragment was amplified by SOE-PCR,then expression vector pET-PPPIL was constructed with pET-PPPI as the carrier,and prepared expression strain BL21(DE3)(pET-PPPIL) which is induced expresses LL-37 fusion protein highly,this results lay a foundation for the purifing LL-37. |
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| Keywords: | polymerase chain reaction human antimicrobial peptide LL-37 protein expression |
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