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尖镰胞菌细胞色素P450 55a1基因超表达载体的构建和水稻日本晴遗传转化
引用本文:吴云华,邓欢.尖镰胞菌细胞色素P450 55a1基因超表达载体的构建和水稻日本晴遗传转化[J].中南民族学院学报(自然科学版),2014(2):32-35.
作者姓名:吴云华  邓欢
作者单位:中南民族大学生命科学学院,武陵山区特色资源植物种质保护与利用湖北省重点实验室,武汉430074
基金项目:国家横向资助项目“RhFGF-FGF-1 135蛋白质的表达纯化工艺优化研究”(HZY11015)
摘    要:要将细胞色素P450 55a1基因克隆到镰刀菌素植物超表达载体pCAMBIA1302中,构建了pCAMBIA1302-cyp55a1-gfp1植物超表达载体,以水稻日本晴为遗传转化的受体对象,通过农杆菌介导侵染方法进行了遗传转化.结果表明:成功构建了细胞色素P450 55a1基因超表达载体,获得了多个细胞色素P450 55a1基因超表达的阳性植株,并以RT-PCR技术分析了阳性植株中细胞色素P450 55a1基因的表达水平.

关 键 词:细胞色素P450  55a1  遗传转化  水稻  尖镰胞菌

Construction of an Over-Expression Vector of Fusarium oxysporium P450 55al Gene and its Genetic Transformation in Rice Nipponbare
Wu Yunhua,Deng Huan.Construction of an Over-Expression Vector of Fusarium oxysporium P450 55al Gene and its Genetic Transformation in Rice Nipponbare[J].Journal of South-Central University for Nationalities(Natural Sciences),2014(2):32-35.
Authors:Wu Yunhua  Deng Huan
Institution:( Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China)
Abstract:The cyp55al full-length gene from Fusarium oxysporium was cloned to construct an over-expression vector pCAMBIA1302-cyp45055al-gfp and the recombinant plasmid was transformed into the rice Nipponbare's callus mediated by Agrobacterium. The results showed that the plasmid pCAMBIA1302-cyp55al was constructed successfully and multiple positive transgenic rice plants with the over-expression of P450 55al were obtained. The expression level of P450 55al in the positive plants was determined by RT-PCR.
Keywords:cytochrome P450 55al  genetic transformation  rice  Fusarium oxysporium
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