| HPLC法测定中江丹参植株不同部位丹参酮Ⅰ、丹参酮Ⅱ的含量 |
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| 引用本文： | 徐辉,李艳,李猛,边清泉,罗娅君,顾冰.HPLC法测定中江丹参植株不同部位丹参酮Ⅰ、丹参酮Ⅱ的含量[J].西南科技大学学报,2011,26(4):92-94. |
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| 作者姓名： | 徐辉 李艳 李猛 边清泉 罗娅君 顾冰 |
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| 作者单位： | 1. 绵阳师范学院化学与化学工程学院,四川绵阳,621000
2. 四川同兴天然植物药业有限公司,四川德阳,610100 |
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| 基金项目： | 四川省科技厅科技支撑计划项目，绵阳师范学院科学研究项目 |
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| 摘 要： | 目的:建立高效液相色谱法同时测定丹参植株中不同部位丹参酮Ⅰ、丹参酮Ⅱ两种脂溶性成分的含量。方法:采DiamonsilTM C18色谱柱(250 mm×4.6 mm,5μm),流动相:甲醇-水(84:16),柱温:35℃,流速为1.0 mL/min,检测波长268 nm。结果:在此色谱条件下2种成分可完全分离。丹参酮Ⅰ、丹参酮Ⅱ的线性范围分别为0.000 51～0.10μg(r=0.999 9),0.002 9～0.058μg(r=0.999 7)。平均回收率丹参酮Ⅰ为98.7%(RSD为1.2%),丹参酮Ⅱ为100.2%(RSD为1.7%)。实验结果可为中江丹参植株地上部位的开发利用提供科学依据。
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| 关 键 词： | 中江丹参 丹参酮Ⅰ 丹参酮Ⅱ 高效液相色谱法 |
Simultaneous Determination of Content of Tanshinone Ⅰand Tanshinone Ⅱ in Different Part of Zhongjiang Salvia Miltiorrhiza by HPLC |
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| XU Hui,LI Yan,LI Meng,BIAN Qing-quan,LUO Ya-jun,GU Bing.Simultaneous Determination of Content of Tanshinone Ⅰand Tanshinone Ⅱ in Different Part of Zhongjiang Salvia Miltiorrhiza by HPLC[J].Journal of Southwest University of Science and Technology,2011,26(4):92-94. |
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| Authors: | XU Hui LI Yan LI Meng BIAN Qing-quan LUO Ya-jun GU Bing |
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| Institution: | XU Hui1,LI Yan1,LI Meng1,BIAN Qing-quan1,LUO Ya-jun1,GU Bing2(1.School of Chemisty and Chemical Engineering,Mianyang Normal University,Mianyang 621000,Sichuan,China,2.Together Cheerful Natural Plant Medicine Industry Limited Company in Sichuan,Deyang 610100,China) |
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| Abstract: | To establish an HPLC for simultaneous determination of two lipid-soluble compounds： Tanshinone Ⅰ , Tanshinone lq in different parts of salvia miltiorrhiza of Zhongjiang. Methods： The column was Diamonsil TM C18 （250 mm× 4. 6 mm,5μm）. The mobile phase consisted of a mixture of methanolwater（84 ： 16）, the column temperature was 35℃ ,the flow rate was 1. 0 mL/rain,the detection wave- length was set up at 268 nm. Results： The linearity was obtained over the ranges of 0. 000 51 - 0.10 p,g （ r =0. 999 9） for Tanshinone Ⅰwith average recovery of 98.7% （ RSD was 1. 2% ）, 0.002 9 - 0.058 μg （ r = 0. 999 7） for Tanshinone Ⅱ with average recovery of 100.2% （ RSD was 1.7% ）. Conclusion： The method is rapid, simple, and reliable, and can be used for simultaneous determination of two lipid-soluble compounds in different parts of salvia miltiorrhiza |
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| Keywords: | Salvia miltiorrhiza of Zhongiiang Tanshinone Ⅰ Tanshinone Ⅱ HPLC |
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