| 十拷贝串联重复laGLP-1基因植物表达载体的构建及对黄瓜遗传转化的研究 |
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| 引用本文： | 赵磊,廖芳,王翠艳,陈苗,魏爱民,杜胜利,马百成,胡晓宇,武志强,王瑞菊,李明刚.十拷贝串联重复laGLP-1基因植物表达载体的构建及对黄瓜遗传转化的研究[J].东北师大学报(自然科学版),2009,41(3). |
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| 作者姓名： | 赵磊 廖芳 王翠艳 陈苗 魏爱民 杜胜利 马百成 胡晓宇 武志强 王瑞菊 李明刚 |
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| 作者单位： | 赵磊,王瑞菊,李明刚,廖芳,陈苗,马百成,胡晓宇,武志强(南开大学生命科学学院分子生物学研究所,生物活性材料教育部重点实验室,天津,300071);王翠艳(内蒙古工业大学生物工程系,内蒙古,呼和浩特,010000);魏爱民,杜胜利(天津市科润黄瓜研究所,天津,300192) |
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| 基金项目： | 国家高科技研究发展计划(863)资助项目，天津市农业重点攻关项目 |
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| 摘 要： | 天然胰高血糖素样肽-1(glucagon-like peptide-1,GLP-1)是含有30个氨基酸的短肽激素,对2型糖尿病具有特殊疗效.以本实验室克隆的长效GLP-1(long acting GLP-1,laGLP-1)基因为基础,构建了十拷贝串联重复laGLP-1的无抗生素选择标记的植物表达载体pX6-laGLP-1,采用农杆菌介导法对黄瓜自交系2M1进行转化,通过PCR及Southern杂交检测分析,最后获得了4株稳定整合十拷贝串联重复laGLP-1的转基因植株.该遗传体系的建立为研发糖尿病食疗型转基因植物或用其生产治疗糖尿病的口服药物奠定了基础.
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| 关 键 词： | 糖尿病 黄瓜 农杆菌介导法 |
Construction of plant expression vector containing ten-tandem repeat laGLP-1 gene and its genetic transformation in cucumber |
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| ZHAO Lei,LIAO Fang,WANG Cui-yan,CHEN Miao,WEI Ai-min,DU Sheng-li,MA Bai-cheng,HU Xiao-yu,WU Zhi-qiang,WAGN Rui-ju,LI Ming-gang.Construction of plant expression vector containing ten-tandem repeat laGLP-1 gene and its genetic transformation in cucumber[J].Journal of Northeast Normal University (Natural Science Edition),2009,41(3). |
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| Authors: | ZHAO Lei LIAO Fang WANG Cui-yan CHEN Miao WEI Ai-min DU Sheng-li MA Bai-cheng HU Xiao-yu WU Zhi-qiang WAGN Rui-ju LI Ming-gang |
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| Institution: | ZHAO Lei1,LIAO Fang1,WANG Cui-yan2,CHEN Miao1,WEI Ai-min3,DU Sheng-li3,MA Bai-cheng1,HU Xiao-yu1,WU Zhi-qiang1,WAGN Rui-ju1,LI Ming-gang1(1.The Key Laboratory for Bioactive Materials of the Ministry of Education,Institute for Molecular Biology,College of Life Sciences,Nankai University,Tianjin 300071,China,2.Bioengineering College of Inner Mongolia University of Technology,Huhehaote 010000,3.Tianjin Kernel Cucumber Research Institute,Tianjin 300192,China) |
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| Abstract: | Glucagon-like peptide-1(GLP-1) is a short peptide incretion of 30 amino acid with the especial treatment of type 2 diabetes.In this study,marker-free expression vector pX6 with a fusion gene of 10 tandem repeated GLP-1 genes named pX6-laGLP-1 was constructed which based on the gene of long acting GLP-1(laGLP-1) cloned in our laboratory.Then it was transformed into cucumber inbreeding line 2M1 by Agrobacterium tumefaciens-mediated transformation.Four transgenic lines with stable integration ten-tandem repeat... |
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| Keywords: | laGLP-1 |
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