| 纳米火棉胶膜自组装细胞色素c的直接电化学研究 |
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| 引用本文： | 秦玉华,周立恒,王岩,马丽,王胜天.纳米火棉胶膜自组装细胞色素c的直接电化学研究[J].东北师大学报(自然科学版),2010,42(1). |
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| 作者姓名： | 秦玉华 周立恒 王岩 马丽 王胜天 |
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| 作者单位： | 东北电力大学化学工程学院,吉林,吉林132012;东北师范大学化学学院,吉林,长春,130024 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 用纳米火棉胶膜将细胞色素c固定在玻碳电极表面,制备了细胞色素c-火棉胶膜修饰电极.吸附在火棉胶膜上的细胞色素c可以与电极发生直接电子传递.在pH=7.0的0.1mol/LPBS缓冲溶液中可得到一对准可逆的细胞色素c的血红素辅基Fe(Ⅲ)/Fe(Ⅱ)电对氧化还原峰,实验求得细胞色素c异相电子传递速率常数k0为65.4μm/s.进一步考察了扫速、溶液pH值等因素对细胞色素c电子传递的影响,并用电化学阻抗法研究了修饰电极的电化学行为.
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| 关 键 词： | 细胞色素c 火棉胶 直接电化学 |
Direct electrochemistry of cytochrome c immobilized in nanoporous collodium |
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| QIN Yu-hua,ZHOU Li-heng,WANG Yan,MA Li,WANG Sheng-tian.Direct electrochemistry of cytochrome c immobilized in nanoporous collodium[J].Journal of Northeast Normal University (Natural Science Edition),2010,42(1). |
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| Authors: | QIN Yu-hua ZHOU Li-heng WANG Yan MA Li WANG Sheng-tian |
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| Abstract: | Cytochrome c was immobilized on glass carbon electrode by collodium.The cytochrome c adsorbed in the collodium film undergoes fast direct electron-transfer reactions,corresponding to Fe~Ⅲ+e~--→Fe~Ⅱ in 0.1 mol/L PBS buffer (pH = 7.0).The heterogeneous electron transfer rate constant (k~0) of cytochrome c was evaluated to be ca.65.4 μm/s.The effect of scan rate,pH and concentration of buffers on the electrochemical behaviors of cytochrome c is studied in detail.Electrochemical property of cytochrome c on modified electrode is investigated by electrochemical impedance spectroscopy. |
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| Keywords: | cytochrome c collodium direct electrochemistry |
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