红松种子休眠的生理生化研究——碳水化合物对离体胚萌发的影响及种子在低温砂藏过程中碳水化合物的变化

红松离体胚在无外加碳源的培养基中,萌发率很低,提供碳源后,体眠种子的离体胚能很好萌发。萌发率受碳水化合物的种类和浓度的影响。低温砂藏能代替外加碳源的作用,红松种子体内碳水化合物,尤其是还原糖的增加直接或间接影响种子生理后熟,是解除种子休眠的重要条件之一。

Physiological and Biochemical Studies on Seed Dormancy of Pinus Koraiensis --Effect of Carbohydrates on Germination of Excised Embryos and Changes of Carbohydrates in Seeds during Stratification

Germination percentage is low when excised embroys of Pinus Koraiensis were cultured in media without the suppy of exogenous carbohydrates. After carbohydrates being provided, excised embroys of dormancy seeds germinated well. Germination percentage is affected by the kinds and concentrations of carbohydrates. Low temperature can replace the role of exogenus carbohydrates. Therefore, the increase of carbohydrates, especially reducing sugars in seeds, is directly or indirectly related to after-ripening of seeds, and is one prerequisites for breaking the seed dormancy.