| 盐酸克伦特罗人工抗原的制备与鉴定 |
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| 引用本文： | 周景明,段倩倩,祁艳华,栗宁,郑伟,王新洲,陈菲,王爱萍.盐酸克伦特罗人工抗原的制备与鉴定[J].郑州大学学报(自然科学版),2014(2):99-103. |
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| 作者姓名： | 周景明 段倩倩 祁艳华 栗宁 郑伟 王新洲 陈菲 王爱萍 |
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| 作者单位： | 郑州大学生命科学学院,河南郑州450001 |
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| 基金项目： | 国家科技支撑计划子课题,编号20111BAK10B01-17;河南省科技攻关项目,编号132102110050. |
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| 摘 要： | 利用重氮化法合成了盐酸克伦特罗的人工抗原,采用SDS-PAGE凝胶电泳法以及紫外扫描法验证了人工抗原偶联成功,并且运用紫外分光光度法计算了人工抗原的偶联比,偶联物CL-BSA和CL-OVA的偶联比分别为27.4∶1.0和12.0∶1.0.用合成的人工抗原CL-BSA免疫Balb/c小鼠获得了良好的效果.
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| 关 键 词： | 盐酸克伦特罗 人工抗原 偶联比 多克隆抗体 |
Synthesis and Identification of the Artificial Antigen of Clenbuterol Hydrochloride |
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| Institution: | ZHOU Jing-ming,DUAN Qian-qian,QI Yan-hua,LI Ning,ZHENG Wei,WANG Xin-zhou,CHEN Fei( 1.School of Life Science,Zhengzhou University,Zhengzhou 450001,China) |
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| Abstract: | In order to obtain the clenbuterol artificial antigens,the hapten CL was conjugated with BSA or OVA by diazotization.The conjugated molecules were identified by SDS-PAGE electrophoresis and UV-spectrophotometer analysis.The ratios of conjugated clenbuterol artificial antigens were calculated by UV-spectrophotometer analysis.The conjugated ratio of CL to BSA was 27.4∶1.0 and 12.0∶1.0 for CL to OVA.Higher serum antibody titer was detected in the mice immunized with the synthetic artificial antigen CL-BSA. |
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| Keywords: | clenbuterol hydrochloride artificial antigen conjugated ratio polyclonal antibody |
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