| 甘草愈伤组织培养及其代谢产物甘草酸的研究 |
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| 引用本文： | 杨会琴,李敬,戴翠萍,崔娜.甘草愈伤组织培养及其代谢产物甘草酸的研究[J].河北师范大学学报(自然科学版),2006,30(3):346-348. |
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| 作者姓名： | 杨会琴 李敬 戴翠萍 崔娜 |
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| 作者单位： | 河北经贸大学,生物科学与工程学院,河北,石家庄,050061 |
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| 摘 要： | 以MS培养基为基础,分别以甘草根、胚轴、子叶为外植体进行培养,考察光照、2,4-D质量浓度、不同激素组合、pH等理化因子对愈伤组织生长的影响,对代谢产物甘草酸进行了HPLC的定量测定.实验表明其中以根的愈伤组织中甘草酸含量最高,为70.2 mg/g;黑暗条件比光照条件更有利于甘草酸合成;以激素组合为2,4-D4.0 mg/L KT0.5 mg/L,pH为5.4时甘草酸含量最高,在此条件下悬浮培养根培养物中甘草酸质量分数为81.6mg/g,比生药增多51.08%.
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| 关 键 词： | 甘草 愈伤组织培养 甘草酸 HPLC |
| 文章编号： | 1000-5854(2006)03-0346-03 |
| 收稿时间： | 2005-10-16 |
| 修稿时间： | 2005-12-20 |
Studies on the Callus Tissue of Glycyrrhiza inflata and the Glycyrrhizic Acid Content |
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| YANG Hui-qin,LI Jing,DAI Cui-ping,CUI Na.Studies on the Callus Tissue of Glycyrrhiza inflata and the Glycyrrhizic Acid Content[J].Journal of Hebei Normal University,2006,30(3):346-348. |
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| Authors: | YANG Hui-qin LI Jing DAI Cui-ping CUI Na |
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| Institution: | College of Bioscience and Project, Hebei Economies and Trade University, Hebei Shijiazhuang 050061, China |
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| Abstract: | Root,hypocotyl and cotyledon of Glycyrrhiza inflata were cultured on MS medium,the effects of some physico-chemical factors on the induction,culture of callus were also investigated,the content of glycyrrhizic acid in the cultures were showed.The results showed:the glycyrrhizic acid content of root cultures was 70.2?mg/g,obviously higher than that of other culture;the glycyrrhizic acid content was higher in dark than that in light;the glycyrrhizic acid content of root suspended culture was 81.6?mg/g,increase by 51.08?% than the crude drug,when hormone association was 2,4-D4.0?mg/L+KT 0.5?mg/L,pH=5.4. |
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| Keywords: | Glycyrrhiza inflata callus culture glycyrrhizic acid HPLC |
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