浓度梯度法筛选华光霉素高产突变株

用华光霉素梯度平皿将华光霉素产生菌进行自然分离，结果表明：梯度法和常规法两种培养基上选出的菌株初筛平均效价分别为１０６．６％和１００％；在常规法中初筛效价比出发菌株高３０％以上的菌株为０，而在梯度法中有４株菌达到或超过这一水平。实验证明：用华光霉素梯度平皿分离不仅使效价的正变频率高于常规分离，而且正变幅度也大于常规分离。

Selection of Nikkomycin high yielding Strains by a Gradient Plate Technique

We suggested a gradient plate technique for screening and selecting high yielding strains of Nikkomycin.The result shows that the average producing potencys of the strains selected by the two methods are 106.6%(in gradient plate )and 100%(in normal plate)respectively.Four strains which produced 30% more Nikkomycin than the parent strain were picked out in gradient plates,but there are no such strains found in normal plates.