| 敬钊缨毛蛛蛛毒对人肝癌细胞BEL-7402细胞增殖、细胞周期和C-myc蛋白的影响 |
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| 引用本文： | 高莉.敬钊缨毛蛛蛛毒对人肝癌细胞BEL-7402细胞增殖、细胞周期和C-myc蛋白的影响[J].河北师范大学学报(自然科学版),2005,29(3):291-294,298. |
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| 作者姓名： | 高莉 |
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| 作者单位： | 河北师范大学,生命科学学院,河北,石家庄,050016 |
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| 基金项目： | 河北省教育厅自然科学基金资助项目(2004110) |
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| 摘 要： | 通过MTT方法可知敬钊缨毛蛛蛛毒对BEL 7402细胞增殖有较强的抑制作用(p<0.05),时效和量效关系良好.IC50为18mg/L.敬钊缨毛蛛蛛毒可以抑制BEL 7402细胞DNA的合成.流式细胞仪检测发现经过蛛毒作用的BEL 7402细胞凋亡率增加,细胞周期阻滞在G0/G1期.WesternBlot方法进一步检测到蛛毒作用BEL 7402细胞72h后,C myc蛋白表达减弱.实验结果表明,敬钊缨毛蛛蛛毒可以抑制人肝癌细胞BEL 7402的增殖和DNA的合成,其药理作用机理可能除了诱导凋亡外主要是使BEL 7402细胞周期相关蛋白C myc表达减弱,导致细胞周期的变化.
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| 关 键 词： | C-myc 人肝癌细胞 细胞增殖 蛛毒 BEL-7402细胞 Western 细胞周期阻滞 MTT方法 流式细胞仪 抑制作用 量效关系 IC50 细胞凋亡 蛋白表达 相关蛋白 诱导凋亡 作用机理 DNA G1期 t方法 检测 合成 减弱 |
| 文章编号： | 1000-5854(2005)03-0291-04 |
Effects of Spider Chilobrachy jingzhao Venom on Human Hepatocelluar Carcinoma BEL-7402 Cell Proliferation,Cell Cycle and C-myc Protein |
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| GAO Li.Effects of Spider Chilobrachy jingzhao Venom on Human Hepatocelluar Carcinoma BEL-7402 Cell Proliferation,Cell Cycle and C-myc Protein[J].Journal of Hebei Normal University,2005,29(3):291-294,298. |
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| Authors: | GAO Li |
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| Abstract: | MTT test showed that the proliferation of BEL-7402 cells in vitro was inhibited significantly by spider Chilobrachy jingzhao venom(p<0.05) in a time-dependent and concentration-dependent manner.IC_(50) was 18?mg/L(48?h).The spider venom could inhibit the DNA synthesis of BEL-7402.Flow cytometric analysis showed that the spider venom induced the apoptosis and increased the proportion of cells in the G_0/G_1 phase of cell cycle.In Western Blot expression of C-myc protein obviously was down-regulated.The result of this experiment showed that spider venom could inhibit the proliferation and DNA synthesis of BEL-7402.The possible mechanisms are to induce cell apoptosis and to down-regulated C-myc to change the cell cycle of BEL-7402 cells. |
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| Keywords: | the venom of spider Chilobrachy jingzhao BEL-7402 cells cell cycle C-myc protein |
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