| 鸡传染性支气管炎病毒M41及疫苗株H52,H120 S1基因的克隆与序列测定 |
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| 引用本文： | 于秀俊,郑海洲,张莉,赵宝华.鸡传染性支气管炎病毒M41及疫苗株H52,H120 S1基因的克隆与序列测定[J].河北师范大学学报(自然科学版),2004,28(2):181-186. |
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| 作者姓名： | 于秀俊 郑海洲 张莉 赵宝华 |
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| 作者单位： | 沧州师范专科学校,生物系,河北,沧州,061001;河北师范大学,生命科学学院,河北,石家庄,050016 |
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| 基金项目： | 河北省教育厅博士基金资助项目(2002214) |
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| 摘 要： | 根据国外已发表的鸡传染性支气管炎病毒(IBV)S1基因序列，设计了一对引物并以RT—PCR特异性扩增出IBV标准强毒株M41和疫苗株H52，H120的S1基因，基因产物大小为1．62kb，与预期相符，对其测定序列后，同源性比较显示M41株与H52，H120的核苷酸序列的同源性分别为99．2％和97．1％，推导的氨基酸序列的同源性分别为98．3％和95．4％，该结果表明IBV M41与疫苗株H52，H120在S1基因上具有高度的同源性。
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| 关 键 词： | IBV S1基因 核苷酸序列 同源性 |
| 文章编号： | 1000-5854(2004)02-0181-06 |
| 修稿时间： | 2003年9月30日 |
Sequencing and Homology Analysis for the S1 Gene of IBV Strain M41,H120 and H52 |
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| YU Xiu-jun,ZHENG Hai-zhou,ZHANG Li,ZHAO Bao-hua.Sequencing and Homology Analysis for the S1 Gene of IBV Strain M41,H120 and H52[J].Journal of Hebei Normal University,2004,28(2):181-186. |
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| Authors: | YU Xiu-jun ZHENG Hai-zhou ZHANG Li ZHAO Bao-hua |
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| Institution: | YU Xiu-jun~1,ZHENG Hai-zhou~2,ZHANG Li~1,ZHAO Bao-hua~2 |
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| Abstract: | One pair of primers for amplifying the S1 gene of avian infectious bronchitis virus(IBV)had been designed,with which the predicted 1.62?kb fragment had been amplified via RT-PCR from IBV strain M41,H120 and H52.The S1 gene of strain M41 was sequenced and compared with vaccine strains such as H52 and H120.The results showed that the homologies of nucleic acids between M41/H52 and M41/H120 were 99.2% and 97.1% and the homologies of amino acids between M41/H52 and M41/H120 were (98.3%) and 95.4%respectively. |
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| Keywords: | IBV S1 gene nucleotide sequence homology |
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