| 拟南芥悬浮培养细胞外钙结合蛋白的初步检测 |
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| 引用本文： | 崔彦伟,陈伟,崔素娟.拟南芥悬浮培养细胞外钙结合蛋白的初步检测[J].河北师范大学学报(自然科学版),2007,31(2):241-243. |
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| 作者姓名： | 崔彦伟 陈伟 崔素娟 |
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| 作者单位： | 河北师范大学,生命科学学院,河北,石家庄,050016 |
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| 基金项目： | 河北省自然科学基金
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河北师范大学校科研和教改项目 |
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| 摘 要： | 钙离子通过与其结合的蛋白质在植物细胞信号转导中起着重要的作用,已知植物细胞外空间钙离子浓度高达毫摩尔级,而与钙结合的胞外蛋白有待进一步的分析研究.采用丙酮沉淀法分别制备了拟南芥悬浮培养细胞壁盐提蛋白及培养介质蛋白,制备的蛋白经SDS-PAGE电泳分离后,电转移至硝酸纤维素膜,45Ca2 覆盖结合,放射自显影,结果表明悬浮细胞外可能存在多条可与钙离子结合的蛋白条带,其分子量约为17,30,40,68 ku,推测这些蛋白可能参与胞外钙离子相关的生物学过程.
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| 关 键 词： | 拟南芥 悬浮培养细胞 细胞外蛋白 45Ca2 覆盖 |
| 文章编号： | 1000-5854(2007)02-0241-03 |
| 修稿时间： | 2006-11-19 |
Detection of Ca2+-binding Proteins in Extracellular Proteins Extracted from Suspension-cultured Cells of Arabidopsis thaliana |
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| CUI Yan-wei,CHEN Wei,CUI Su-juan.Detection of Ca2+-binding Proteins in Extracellular Proteins Extracted from Suspension-cultured Cells of Arabidopsis thaliana[J].Journal of Hebei Normal University,2007,31(2):241-243. |
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| Authors: | CUI Yan-wei CHEN Wei CUI Su-juan |
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| Institution: | College of Life Science,Hebei Normal University,Hebei Shijiazhuang 050016,China |
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| Abstract: | Calcium plays important roles in cell signal transduction,and Ca2 -binding proteins participate in various activities.There is much Ca2 in extracellular space than that in cytoplasm,so the researches on extracellular Ca2 -binding proteins has an important meaning.First,extracellular proteins were prepared from the suspension-cultured cells of Arabidopsis thaliana,then separated by SDSPAGE and transferred to nitrocellulose membranes.The membranes were blotted with45CaCl2,and then exposed to phosphimager.Four protein bands each with molecular weight about 17,30,40,68 ku were shown.These proteins may function extracellularly with Ca2 . |
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| Keywords: | Arabidopsis thaliana suspension-cultured cell extracellular proteins 45Ca2 overlay |
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