| 超氧物岐化酶提纯研究 |
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| 引用本文： | 张来群,高秀蕊.超氧物岐化酶提纯研究[J].河北师范大学学报(自然科学版),1994,18(4):74-78,83. |
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| 作者姓名： | 张来群 高秀蕊 |
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| 作者单位： | 河北师范大学生物系，河北师范大学化学系 |
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| 摘 要： | 采用氯仿—乙醇提取液盐析、丙酮沉淀及ＤＥ（３２）纤维素柱层析方法，从牛红细胞中分离得到纯品铜锌超氧物歧化酶（Ｃｕ、Ｚｎ—ＳＯＤ）．纯化的酶经聚丙烯酰胺凝胶电泳为一条蛋白带，活性染色在蛋白带的位置上呈现表示酶活性的亮斑，比活力为每毫克２４８７３单位，原子吸收测Ｃｕ、Ｚｎ含量及紫外吸收光谱测定结果制备样与标准ＳＯＤ样相同．
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| 关 键 词： | 牛红细胞 超氧化岐化酶 纯化 |
Purification of Cu-Zn Superoxide Dismutase |
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| Zhang Laiqun,Wang Youai,Gao Xiurui.Purification of Cu-Zn Superoxide Dismutase[J].Journal of Hebei Normal University,1994,18(4):74-78,83. |
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| Authors: | Zhang Laiqun Wang Youai Gao Xiurui |
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| Institution: | Zhang Laiqun;Wang Youai;Gao Xiurui |
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| Abstract: | Cu-Zn Superoxide Dismutase was purified from bovin erythrocytes by treatment with mixtures of chloroform and ethanol, following precipitation withK2HPO4. acetone and chromotagraphy of DEAE- 32 column. The purified enzymeshowed one protein band by PAGE and was active at this protein band by activity dye.It's specific activity was 24873 unite per mg. It is indicated that the purified SOD is thesame as the standard SOD through ultraviolet absorption spectra and quantitation ofCopper and Zinc by atomic absorption spectrophotometry. |
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| Keywords: | Cu-Zn superoxide dismutase bovin erythrocytes purification |
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