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| 嗜水气单胞菌的分离培养及鉴定 | |
| 引用本文： | 李楠,孙讳敏,田莉瑛,李晓玥,申红旗,王风敏,李全振,曹杰英,赵宝华.嗜水气单胞菌的分离培养及鉴定[J].河北师范大学学报(自然科学版),2009,33(2). |
| 作者姓名： | 李楠 孙讳敏 田莉瑛 李晓玥 申红旗 王风敏 李全振 曹杰英 赵宝华 |
| 作者单位： | 1. 河北化工医药职业技术学院制药工程系,河北石家庄050026;河北师范大学生命科学学院,河北石家庄050016 2. 河北化工医药职业技术学院制药工程系,河北石家庄,050026 3. 河北师范大学生命科学学院,河北石家庄,050016 4. 河北省水产局病害监测站,河北石家庄,050011 5. 河北省水产局病当监测站,河北石家庄,050011 |
| 摘 要： | 从患腹水病褐牙鲆体内分离致病菌,通过生理生化鉴定方法对该菌进行了鉴定.根据Genbank 上报道的嗜水气单胞菌标准株的16s rDNA序列以及致病性嗜水气单胞菌所含有的气溶素(aer)基因没汁了2对引物,对所分离到的2株疑似菌以及3株标准株进行PCR扩增,均能够扩增出目的条带,经测序后,该菌的16s rDNA的序列、aer序列与标准株同源性分别达到99.8%和95%,与常规细菌鉴定方法所得结果一致,证明该菌为嗜水气单胞菌. |
| 关 键 词： | 嗜水气单胞菌 分离 鉴定 腹水 |
The Isolation and Identification of Aeromonas hydrophila |
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| LI Nan,SUN Yi-min,TIAN Li-ying,LI Xiao-yue,SHEN Hong-qi,WANG Feng-min,LI Quan-zhen,CAO Jie-ying,ZHAO Bao-hua.The Isolation and Identification of Aeromonas hydrophila[J].Journal of Hebei Normal University,2009,33(2). | |
| Authors: | LI Nan SUN Yi-min TIAN Li-ying LI Xiao-yue SHEN Hong-qi WANG Feng-min LI Quan-zhen CAO Jie-ying ZHAO Bao-hua |
| Institution: | 1.Department of Pharmaceutical Engineering;Hebei Chemical Engineering and Pharmaceutical College;Hebei Shijiazhuang 050026;China;2.College of Life Science;Hebei Normal University;Hebei Shijiazhuang 050016;3.Department of Diseases Supervision;Hebei Aquatic Product Office;Hebei Shijiazhuang 050011;China |
| Abstract: | The Aeromonas hydrophila strains were isolated from the sick Paralichthys olivaceus.The typical symptom was observed through clinical diagnosis and pathological anatomy.Biochemical experimental results showed that this bacteria could be identified as Aeromonas hydrophila.This bacteria was also detected by using polymerase chain reaction based on the aer gene and the 16s rDNA sequence of Aeromonas hydrophila reported on Genbank.The result of the molecular detection was consistent with the biochemical detecti... |
| Keywords: | 16s rDNA |
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