利用PCR-DGGE技术鉴定硝基甲苯类物质降解菌株

为鉴定光催化-厌氧好氧固定化微生物方法降解2,6-DNT和4-MNT的优势菌株,取连续运行40d的厌氧好氧反应器出入口的微生物样品.采用PCR-DGGE技术分离微生物,得到对2,6-DNT和4-MNT具有良好降解特性的优势菌种;发现厌氧反应器内不同位置微生物种类差别很大,好氧反应器内差别很小;反应器内有5种优势菌株:Delta proteobacterium,Pseudomonas stutzeri,Pseudomonas trivialis,Burkholderia cenocepa-ciaand Chryseobacterium sp.AKB-2008-VA6和1个未知菌株,以兼性厌氧和好氧方式生存.反应器运行期间,水质指标良好.

Determination on High Effective Strains of Degrading Nitrotoluene Using PCR-DGGE

To identify the dominant degrading 2,6-dinitrotoluene(2,6-DNT) and 4-mononitrotoluene(4-MNT) bacteria in the photocatalysis-anaerobic-aerobic progress, the microbe samples were collected individually from the inlet and outlet of 40 days running anaerobic-aerobic reactor. The measures of polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) were cooperatively used to segregate microbes in the photocatalysis-anaerobic-aerobic microbial community for obtaining the dominant efficiently degrading 2,6-dinitrotoluene and 4-mononitrotoluene bacteria. It is found that the difference of microbe types is large at different locations in anaerobic reactor, but is less in aerobic reactor. There are five dominant microbes: Delta proteobacterium,Pseudomonas stutzeri, Pseudomonas trivialis,Burkholderia cenocepaciaand Chryseobacterium sp. AKB-2008-VA6 and one unknown microbes. The dominant microbes are facultative anaerobic-aerobic. The water quality is good in 40 days reactor operation.