苦绳中木脂素类化学成分的分离与结构鉴定

为明确苦绳Dregea sinensis Hemsl中的主要化学成分,采用AB-8大孔吸附树脂、硅胶、Sephadex LH-20葡聚糖凝胶和反相制备液相等色谱方法从苦绳中首次分离得到5个木脂素类化合物. 通过一维和二维核磁共振波谱、有机质谱等方法确定它们分别为4',4"-二羟基-3,3',3",5,5',5"-六甲氧基-7,9':7',9-二环氧-4,8"-氧-8,8'-倍半新木脂素-7",9"-二醇(1),4',4"-二羟基-3,3',3",5,5'-五甲氧基-7,9':7',9-二环氧-4,8"-氧-8,8'-倍半新木脂素-7",9"-二醇(2),(+)-异落叶松树脂醇(3),赤式-愈创木酚基甘油基-β-O-4'-松柏醇(4),diasyringaresinol (5). 

Isolation and Structural Identification of Lignans from Dregea sinensis Hemsl

In order to study the chemical constituents of Dregea sinensis Hemsl, five lignans were isolated from the roots of Dregea sinensis Hemsl by AB-8 macroporous resin column, silica gel column, Sephadex LH-20 column and semi-preparative HPLC. On the basis of 1D and 2D NMR data and MS analyses, their structures were identified as 4',4"-dihydroxy-3,3',3",5,5',5"-hexamethoxy-7,9':7',9-diepoxy-4,8"-oxy-8,8'-sesquineolignan-7",9"-diol (1), 4',4"-dihydroxy-3,3',3",5,5'-pentamethoxy-7,9':7',9-diepoxy-4,8"-oxy-8,8'-sesquineo-lignan-7",9"-diol (2), (+)-isolariciresinol (3), erythro-guaiacylycerol-β-O-4'-coniferyl alcohol (4), diasyringaresinol (5). These compounds were isolated from this plant for the first time.