| 基于单细胞凝胶电泳法的不同烷化剂导致DNA损伤的比较研究 |
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| 引用本文： | 陈薛钗,郑晓彤,崔博,罗云敬,钟儒刚.基于单细胞凝胶电泳法的不同烷化剂导致DNA损伤的比较研究[J].北京理工大学学报,2015,35(S1):45-49. |
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| 作者姓名： | 陈薛钗 郑晓彤 崔博 罗云敬 钟儒刚 |
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| 作者单位： | 北京工业大学 生命科学与生物工程学院, 北京 100124;北京市环境与病毒肿瘤学重点实验室, 北京 100124,北京工业大学 生命科学与生物工程学院, 北京 100124;北京市环境与病毒肿瘤学重点实验室, 北京 100124,北京工业大学 生命科学与生物工程学院, 北京 100124;北京市环境与病毒肿瘤学重点实验室, 北京 100124,北京工业大学 生命科学与生物工程学院, 北京 100124;北京市环境与病毒肿瘤学重点实验室, 北京 100124,北京工业大学 生命科学与生物工程学院, 北京 100124;北京市环境与病毒肿瘤学重点实验室, 北京 100124 |
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| 基金项目： | 国家教育部博士后科学基金资助项目(2012M510302) |
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| 摘 要： | 从单细胞水平考察单官能团和双官能团烷化剂引起DNA损伤的差异.采用单细胞凝胶电泳法检测并比较单官能团烷化剂甲基亚硝基脲与双官能团烷化剂尼莫司汀在不同浓度下对DNA的损伤情况.研究结果表明药物浓度与彗星尾部DNA含量之间存在线性关系.为了检测DNA交联损伤,选择加入断裂剂作为对照,结果显示双官能团烷化剂尼莫司汀能引起显著的DNA交联.本文从细胞毒性作用和DNA损伤水平考察了烷化剂的毒性,为阐明烷化剂的致癌和抗癌机制提供理论基础.
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| 关 键 词： | DNA损伤 烷化剂 尼莫司汀 单细胞凝胶电泳 DNA交联 |
| 收稿时间： | 2015/3/20 0:00:00 |
Comparison of DNA Damage Induced by Different Alkylating Agent by Single Cell Gel Electrophoresis Assay |
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| CHEN Xue-chai,ZHENG Xiao-tong,CUI Bo,LUO Yun-jing and ZHONG Ru-gang.Comparison of DNA Damage Induced by Different Alkylating Agent by Single Cell Gel Electrophoresis Assay[J].Journal of Beijing Institute of Technology(Natural Science Edition),2015,35(S1):45-49. |
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| Authors: | CHEN Xue-chai ZHENG Xiao-tong CUI Bo LUO Yun-jing and ZHONG Ru-gang |
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| Institution: | College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China;Beijing Key Laboratory of Environment and Viral Oncology, Beijing 100124, China,College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China;Beijing Key Laboratory of Environment and Viral Oncology, Beijing 100124, China,College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China;Beijing Key Laboratory of Environment and Viral Oncology, Beijing 100124, China,College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China;Beijing Key Laboratory of Environment and Viral Oncology, Beijing 100124, China and College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China;Beijing Key Laboratory of Environment and Viral Oncology, Beijing 100124, China |
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| Abstract: | Toxic effects of alkylating agents on cell survival and DNA damage was investigated on a single cell level. In this study, single cell gel electrophoresis assay (SCGE) was used to detect and compare DNA damages induced by monofunctional and bifunctional alkylating agents under different concentrations. Linear positive correlation between drug concentration and DNA damages has been demonstrated. To further investigate the possible induction of DNA crosslinking by ACNU, DNA cleavage reagent was used, and results show that ACNU can cause significant DNA crosslinks. The result have provided theoretical basis for clarifying carcinogenic and anti-cancer mechanisms of alkylating agents. |
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| Keywords: | DNA damage alkylating agent nimustine SCGE DNA crosslinking |
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