| 氧化石墨烯对DNA荧光分子探针FRET效应的研究 |
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| 引用本文： | 张从晓,吕雪飞,庆宏,邓玉林.氧化石墨烯对DNA荧光分子探针FRET效应的研究[J].北京理工大学学报,2015,35(S1):123-127. |
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| 作者姓名： | 张从晓 吕雪飞 庆宏 邓玉林 |
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| 作者单位： | 北京理工大学 生命学院, 北京 100081,北京理工大学 生命学院, 北京 100081,北京理工大学 生命学院, 北京 100081,北京理工大学 生命学院, 北京 100081 |
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| 基金项目： | 国家自然科学基金资助项目(21275019);国家重大科学仪器设备开发专项基金资助项目(2012YQ04014005) |
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| 摘 要： | 研究了氧化石墨烯的浓度、氧化石墨烯与DNA分子荧光探针的反应温度、DNA链长度对FRET效应的影响.研究表明,氧化石墨烯的浓度、氧化石墨烯与DNA分子荧光探针的反应温度、DNA链长度对FRET效应都有着重要的影响.氧化石墨烯浓度越大,其对DNA分子探针的荧光猝灭效率越高;氧化石墨烯与DNA分子荧光探针的反应温度越高,淬灭效率越高;DNA链越长,氧化石墨烯淬灭的效率越低.因此,氧化石墨烯的浓度、氧化石墨烯与DNA分子荧光探针的反应温度、DNA链长度对FRET效应都应该是设计生物传感器时考虑的因素.
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| 关 键 词： | 氧化石墨烯 荧光共振能量转移 分子探针 |
| 收稿时间： | 2015/3/20 0:00:00 |
FRET Effect on DNA Fluorescent Molecular Probe by Graphene Oxide |
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| ZHANG Cong-xiao,L&#; Xue-fei,QING Hong and DENG Yu-lin.FRET Effect on DNA Fluorescent Molecular Probe by Graphene Oxide[J].Journal of Beijing Institute of Technology(Natural Science Edition),2015,35(S1):123-127. |
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| Authors: | ZHANG Cong-xiao L&#; Xue-fei QING Hong and DENG Yu-lin |
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| Institution: | 1. Department of Electronic Engineering, School of Information Science and Technology, Beijing Institute of Technology, Beijing 100081, China,1. Department of Electronic Engineering, School of Information Science and Technology, Beijing Institute of Technology, Beijing 100081, China,1. Department of Electronic Engineering, School of Information Science and Technology, Beijing Institute of Technology, Beijing 100081, China and 1. Department of Electronic Engineering, School of Information Science and Technology, Beijing Institute of Technology, Beijing 100081, China |
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| Abstract: | Fluorescence resonance energy transfer (FRET) effect of graphene oxide on DNA fluorescent molecular probe is a very important factor of DNA the biosensor, which is related to the detection sensitivity of graphene oxide based DNA biosensors. The effects of the concentration of graphene oxide, the reaction temperature, and the length of DNA on FRET were investigated. The results show that the concentration of graphene oxide, the reaction temperature, and the length of DNA play significant roles on FRET effects. The fluorescence quenching efficiency of DNA molecule probe was improved with the increasing of graphene oxide concentration and the reaction temperature, with the decreasing of the length of DNA. Therefore, in order to get the excellent biosensor, the concentration of graphene oxide, the reaction temperature, and the length of DNA should be taken into account in the design of graphene oxide DNA biosensor. |
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| Keywords: | graphene oxide fluorescence resonance energy transfer (FRET) molecular probe |
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