发菜中红色蛋白的研究

研究发菜中一种红色蛋白(Rx)的分离纯化及其光谱学性质.采用超声法破碎发菜细胞,盐析法初步纯化藻胆蛋白,利用DEAE-DE52交换介质得到藻胆蛋白纯品;测定室温和低温(液氮)下的荧光激发光谱和发射光谱.Rx的吸收峰在520 nm,室温与低温下的荧光发射峰分别在625和630 nm,室温与低温下的荧光激发峰分别在615和610 nm,室温下,Rx在藻胆体中的荧光上升时间和荧光寿命分别为495和2 200 ps.结果表明,Rx既可以把它吸收的光能传递给藻蓝蛋白(PC),再经由PC传递给别藻蓝蛋白(APC),又可以直接将能量传递给APC.

Study on the Red Protein of Nostoc Flagelliforme

The purification and character of spectrum of red protein(Rx) of Nostoc flagelliforme was studied in the paper.First,the cell of Nostoc flagelliforme was broken up with ultrasonic treatment,then the phycobiliprotein was preliminary purified with salting out and purified by chromatography with DEAE-DE52 as a medium.The fluorescence spectra were detected at room temperature and liquid nitrogen condition.The absorption peak of Rx was at 520 nm,the peak of fluorescence emission at room temperature and liquid nitrogen temperature were at 625 and 630 nm respectively,the peak of fluorescence excitation at room temperature and liquid nitrogen temperature were at 615 and 610 nm respectively,the rising time of fluorescence and life time were 495,2 200 picosecond respectively.With the results above,it could be concluded that the energy absorbed by Rx could transfer to PC then via PC to APC,and could also transfer to APC directly.