HPLC-UV-ELSD同时检测新型催泪喷射器中的合成辣椒素与吐温20

为检测警用催泪喷射器中合成辣椒素和吐温20的质量浓度，建立了一种紫外与蒸发光散射检测器联用的高效液相色谱方法（HPLC-UV-ELSD）.其中紫外检测器检测合成辣椒素（SC）、蒸发光散射检测器检测吐温20.检测条件为:流动相为甲醇:水（80∶20），流速1 mL/min，紫外检测波长280 nm，柱温28℃；蒸发光散射检测器空气载气流量2.5 L/min，漂移管温度为60℃，雾化载气流压力设置为0.5 MPa.在合成辣椒素质量浓度为196~980 μg/mL范围内，吐温20在质量浓度为212~1 060 μg/mL范围内呈良好的线性关系（R2>0.999），回收率均在90%~110%范围内.本方法已成功应用于实际警用催泪喷射器填充剂的检测. 

Simultaneous Determination of Tween 20 and Synthetic Capsaicin for New Type of SC-Sprays by HPLC-UV-ELSD

To simultaneous determination of synthetic capsaicin (SC) and Tween 20 in SC-sprays for police use, a HPLC-UV-ELSD method was developed. The SC and Tween 20 were successively detected by the UV detector and ELSD detector combined in one HPLC system. The chromatographic conditions were optimized as follow:the mobile phase was methanol and water (80:20), flow rate was 1.0 mL/min, UV wavelength was 280 nm, and the column temperature was set at 28℃. The flow rate of air of ELSD was maintained at 2.5, the pressure was 0.5 MPa, and the temperature of tube was kept at 60℃. The calibration curves built with the mixing standard were linear in the ranges of 196~980 μg/mL for SC,and 212~1 060 μg/mL (R2>0.999) for Tween 20. Results show that, the recoveries can be obtained in the range of 90%~110%. This developed method has been demonstrated to be suitable for determining the real filling agents in SC-sprays for police use.