龙血素B在模拟失重效应大鼠体内的分布研究

研究传统名贵傣药龙血竭中有效成分龙血素B在大鼠体内重要组织的分布情况，同时探讨长期模拟失重效应对龙血素B分布的潜在影响.将SD大鼠分为正常重力组和模拟失重效应组，采用21 d大鼠尾悬吊方法模拟长期失重效应，单次灌胃给予大鼠25 mg/kg龙血素B，于给药1 h后收集心、肝、脾、肺、肾、胃、肠、脑、睾丸和骨骼肌10种组织，采用HPLC-MS/MS方法测定各组织中龙血素B质量分数.在正常重力组中，龙血素B在肝、胃分布较多，其次是肾、肠、心、脾、骨骼肌和肺，脑中最少.与正常重力组相比，模拟失重效应组大鼠肝中龙血素B的质量分数显著升高47.7%（p<0.05），脑中质量分数上升5.4倍（p<0.05）；在肠和肾中质量分数显著下降52.7%和22.0%（p<0.05）；在大鼠胃、心、肺、脾、睾丸和骨骼肌中质量分数无显著变化.龙血素B的分布具有明显组织特异性，长期模拟失重效应显著改变龙血素B在大鼠肝、肠、脑、肾等重要组织的分布. 

Tissue Distribution of Loureirin B in Rats Under Simulated Microgravity Effect

To study the tissue distribution of Loureirin B, an active ingredient of traditional Chinese medicine-dragons blood, and the potential effects of long-term simulated microgravity effect on drug distribution. SD rats were randomly divided into normal gravity group and simulated microgravity group. And rats were tail-suspended for 21 days to simulate microgravity effect. Single dose of 25 mg/kg Loureirin B was orally administered to all rats respectively. Heart, liver, spleen, lung, kidney, stomach, intestine, brain, testis and skeleton muscle samples were collected at 1 h postdosing. Concentration of Loureirin B in samples was determined by HPLC-MS/MS method to observe the effect of simulated microgravity effect on Loureirin B distribution in vivo. The results show that, Loureirin B is distributed significantly in the liver and stomach, followed by kidney, intestine, heart, spleen, skeletal muscle, lungand brain. The least distribution is found in brain. Compared with the normal gravity group, Loureirin B in the liver from the simulated microgravity rats is obviously increased by 47.7% (p<0.05), and the content of Loureirin B in rat brain is increased by 5.4-fold (p<0.05).Loureirin B is decreased significantly in the intestine and kidney with a decrease by 52.7% and 22.0% respectively (p<0.05).There is no dramatic difference in Loureirin B level of stomach, heart, lung, spleen, testis and skeletal muscle. Loureirin B distribution shows obvious tissue specificity. Loureirin B distribution in liver, intestine, brain and kidney of rats is evidently changed by long-term simulated microgravity effect.