基于核酸适配体的荧光分析法定量检测MUC1粘蛋白

MUC1粘蛋白是一种高糖化、高分子量的糖蛋白，其在乳腺癌细胞中高度异常表达，其特异性高于组织多肽抗原，敏感性高于癌胚抗原，因此MUC1在乳腺癌诊断中具有很高的临床应用价值，而建立高灵敏的MUC1蛋白定量检测方法对临床诊断具有重要的意义.该研究建立了基于核酸适配体-滚环扩增（RCA）和氧化石墨烯-荧光共振能量转移（GO-FRET）技术的MUC1黏蛋白定量检测技术，实现了MUC1粘蛋白准确、灵敏的定量检测.结果表明，该方法定量检测线性范围为50~1 000 pg/mL，检测限为28.05 pg/mL，定量限为45.57 pg/mL，在人血样品中的回收率为96%~104%.

Quantitative Detection of MUC1 Protein by Aptamer-Based Fluorescence Analysis

MUC1 is a highly glycosylyted and large-molecular-weight glycoprotein, mainly expressed highly abnormal in breast cancer cells. Its specificity is higher than tissue polypeptide antigen, and its sensitivity is higher than cancer embryo antigen. Therefore, MUC1 possesses great clinical value in the diagnosis of breast cancer, and the development of a highly sensitive detecting method for MUC1 protein plays a more important role for clinical diagnosis. In the present study, sensitive detecting method for MUC1 was based on nucleic acid aptamer-rollong circle amplification (RCA) and graphene oxid-fluorescence resonance energy transfer (GO-FRET). The results show that, the linear range of quantitative detection can reach 50~1 000 pg/mL, and the limit of detection is 28.05 pg/mL, the limit of quantitation is 45.57 pg/mL. The recovery of MUC1 in human serum can be ranged from 96% to 104%.