铁死亡在胶质瘤治疗领域的作用

爱拉斯汀（Erastin）通过抑制SLC7A11摄取胱氨酸/谷氨酸的能力引起铁死亡（一种非细胞凋亡，坏死性凋亡和自噬形式的细胞死亡方式）.然而，铁死亡调节的分子机制仍然很大程度上未知.研究铁死亡对胶质细胞的影响对胶质瘤的治疗具有重要作用.本文利用MTS法和细胞计数器分别对加药组细胞和辐照后的细胞进行计数.加入不同浓度的Erastin培养野生型胶质瘤细胞U87MG和P53突变型胶质瘤细胞U251MG，本文发现U87MG对Erastin敏感.伽马射线不能进一步促进Erastin引起的铁死亡.具有抗氧化作用的传统中药龙血竭能有效抑制Erastin引起的铁死亡.总之，本文发现Erastin在胶质瘤化疗领域的重要作用，同时龙血竭在抑制铁死亡和保护健康细胞方面具有重要作用.

The Role of Ferroptosis in the Field of Glioma Treatment

Erastin causes ferroptosis (this form of iron-dependent cell death is distinct from apoptosis, necroptosis, and autophagy) by inhibiting the activity of SLC7A11 to acquire cystine/glutamate. However, the molecular mechanism of iron death regulation remains largely unknown. Studying the effects of ferroptosis on glial cells plays an important role in the treatment of glioma. The cells of the dosing group and the cells after irradiation were counted by MTS method and MILLIPORE Scepter hand-held automated cell counter, respectively. Wild type glioma cells U87MG and P53 mutant glioma cells U251MG were cultured with different concentrations of Erastin. Analysis results show that, the U87MG is sensitive to Erastin. Besides, Gamma rays can not further promote the ferroptosis caused by Erastin. Traditional Chinese medicine Dragon Blood with anti-oxidation can effectively inhibit ferroptosis caused by Erastin. In summary, the Erastin can play an important role in the field of glioma chemotherapy, and the Dragon''s blood can play an important role in inhibiting iron death and protecting healthy cells.