大肠杆菌核酸适配体筛选的研究

由于传统微生物检测存在步骤繁琐、耗费时间、成本较高等缺点，因此研发快速有效检测微生物的新技术新方法显得尤为必要.本文利用whole-cell SELEX技术，进行了大肠杆菌活细胞核酸适配体的筛选，对单链DNA的制备鉴定、每轮筛选核酸文库的检测、核酸适配体的扩增克隆等内容进行了研究，在筛选过程中成功制备了单链DNA，并对整个筛选过程的核酸适配体库进行监测，保证了筛选的顺利进行，并对核酸适配体库进行了扩增和克隆，最终获得靶向大肠杆菌的核酸适配体序列，这将有助于基于核酸适配体的微生物检测新方法的建立.

Aptamer Selecion for Escherichia Coli

The traditional methods of microbial detection usually have shortcomings including cumbersome steps,consuming time and high cost,so developing rapid and effective detection of new technologies and methods for microbial detection is particularly necessary.In this paper,whole-cell SELEX technique was used to screen the aptamers of living cells of Escherichia coli.It is investigated of the preparation of single-stranded DNA,the detection of nucleic acid library for each round of screening,and the cloning of aptamers of nucleic acid.Single stranded DNA was successfully prepared in the screening process,and the aptamer library was monitored in each cycle of the selection to ensure the quantity of the screening.The aptamers was amplified and cloned to obtain the nucleic acid sequence targeted to Escherichia coli.This will facilitate the establishment of a new method for microbial detection based on nucleic acid aptamers.