| 水仙花中绿原酸含量的测定 |
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| 引用本文： | 罗跃中,李忠英,温拥军.水仙花中绿原酸含量的测定[J].邵阳学院学报(自然科学版),2008,5(2):97-99. |
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| 作者姓名： | 罗跃中 李忠英 温拥军 |
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| 作者单位： | 湖南化工职业技术学院,湖南,株洲,412004 |
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| 摘 要： | 目的：对水仙的花、叶及根等部位的绿原酸含量进行检测．方法：采用70％乙醇超声提取样品3次，用紫外分光光度法测定其含量结果：确定了绿原酸的最大吸收波长为331nm，以绿原酸的吸光度和浓度作标准曲线，得回归方程：y=52103x＋0．0047，1R^2=0．9994；测定水仙的花、叶及根的绿原酸含量分别为2．70％、0．90％和0．35％，加样回收率为9989％结论：该方法操作简便、结果准确可靠且稳定性好．
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| 关 键 词： | 水仙 绿原酸 分光光度法 含量测定 |
Determination of Chlorogenic Acid In Narcissus |
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| LUO Yue-Zhong,LI Zhong-Ying,WENG Yong-Jun.Determination of Chlorogenic Acid In Narcissus[J].Journal of Shaoyang University:Science and Technology,2008,5(2):97-99. |
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| Authors: | LUO Yue-Zhong LI Zhong-Ying WENG Yong-Jun |
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| Institution: | LUO Yue-Zhong, LI Zhong-Ying, WENG Yong-Jun (Hunan Chemical Industry Vocation Technology Institute , HuNan Zhuzhou 412004 ) |
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| Abstract: | Objective：Determination of narcissus flower, leaves and roots of chlorogenic acid. Methods：Uses 70% ethyl alcohol supersonic extraction sample 3 times,Determines its content with the UV spectrophotometry.Results Had determined the chlorogenic acid maximum absorption wavelength is 33Into,Make the standard curve by the chlorogenic acid Absorbance and concentration, Regression equation： y = 52.103x ＋0.0047, R^2=0.9994;Determination of narcissus flower, leaves and roots of chlorogenic acid content of 2.70% ,0.90%and0.35% ,Canadian recovery was 99.89%.RSD is 0.16%. Conclusion：The method operation is simple, result is accurate and reliable, and the stability is good. |
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| Keywords: | Narcissus Chlorogenic acid Spectrophotometry Determination |
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