| At3g13600截短型蛋白的原核表达载体的构建 |
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| 引用本文： | 王小兰,周宇,彭慧峰,刘顺枝,田长恩.At3g13600截短型蛋白的原核表达载体的构建[J].广州大学学报(自然科学版),2011,10(3):37-40. |
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| 作者姓名： | 王小兰 周宇 彭慧峰 刘顺枝 田长恩 |
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| 作者单位： | 广州大学生命科学学院,广东广州,510006 |
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| 基金项目： | 国家自然科学基金项目，广州市科技计划项目，广州市属高校科技计划项目 |
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| 摘 要： | 拟南芥基因At3g13600编码一种钙调素结合蛋白,序列分析表明,该基因cDNA序列全长1 818 bp,编码一个具有606个氨基酸残基的多肽,推测分子量为68.9 kD;在At3g13600蛋白的N端存在IQ钙调素结合构象.为了从实验上进一步研究该蛋白的钙调素结合特性,通过逆转录聚合酶链式反应( RT-PCR)扩增含...
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| 关 键 词： | 钙调素结合蛋白 IQ基序 表达载体构建 |
Construction of the recombinant prokaryotic expression plasmids contain truncated form of At3g13600 protein |
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| WANG Xiao-lan,ZHOU Yu,PENG Hui-feng,LIU Shun-zhi,TIAN Chang-en.Construction of the recombinant prokaryotic expression plasmids contain truncated form of At3g13600 protein[J].Journal og Guangzhou University:Natural Science Edition,2011,10(3):37-40. |
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| Authors: | WANG Xiao-lan ZHOU Yu PENG Hui-feng LIU Shun-zhi TIAN Chang-en |
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| Institution: | WANG Xiao-lan,ZHOU Yu,PENG Hui-feng,LIU Shun-zhi,TIAN Chang-en(School of Life Sciences,Guangzhou University,Guangzhou 510006,China) |
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| Abstract: | Arabidopsis gene At3g13600 encoded a calmodulin-binding protein.Sequence analysis showed that the full length cDNA was 1 818 bp and encoded a polypeptide containing 606 amino acid residues,its molecular weight is about 68.9 kD.Amino acid sequence of the deduced protein revealed the existence of IQ calmodulin-binding motif at the N-terminus.To further study the calmodulin binding characteristics of the protein,the coding domain of different length cDNAs were reverse amplified by polymerse chain reaction(PCR)... |
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| Keywords: | calmodulin-binding protein IQ motif expression vector construction |
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