| 芥篮叶超氧化物歧化酶的分离提纯 |
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| 引用本文： | 吴丹奇,张尔贤.芥篮叶超氧化物歧化酶的分离提纯[J].汕头大学学报(自然科学版),1991,6(1):73-77. |
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| 作者姓名： | 吴丹奇 张尔贤 |
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| 摘 要： | 本文建立硫酸铵分级沉淀、Sephadex G-100层析,DEAE 纤维素(DE-52)拄层析方法从芥篮(B.alboglabra Baileg)叶肉中提纯超氧化物歧化酶,纯化倍数45.2,比活力97.3u/mg-pr(肾上腺素测定).南 DEAE-DE52层折获得的 SOD 活性部分,按 Weber与 Osborn 法作 SDS-PAGE,呈现单一斑带,分子量为13,000.此酶对热稳定性不高,70℃保温90min,活力全部丧失.纯酶液的最大吸收波长为204nm,芥篮叶中有四种 SOD同工酶.
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| 关 键 词： | 芥篮 超氧化物歧化酶 提纯 |
Purifiation of a Superoxide Dismutase from B.alboglabra Baileg |
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| Wu Danqi Zhang Erxian Li Wenfang Luo Hanqi.Purifiation of a Superoxide Dismutase from B.alboglabra Baileg[J].Journal of Shantou University(Natural Science Edition),1991,6(1):73-77. |
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| Authors: | Wu Danqi Zhang Erxian Li Wenfang Luo Hanqi |
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| Institution: | Wu Danqi Zhang Erxian Li Wenfang Luo Hanqi |
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| Abstract: | A purified superoxide dismutase(SOD)from B.alloglabra Baileg.has beeh obtained by using a combined extraction procedure of graded(NH_4)_2SO_4 precipita- tion,gel filtration on sephadex G-100 followed by ion-exchange chromatography on DEAE cellulose DE-52.The pufied enzyme showed a single protein band on polyacrylamind gel electrophoresis The specifici activity of the purified enzyme is 97.3 u/mg-pro.The molecular weight of the enzyme,as determined by PAGE-SDS,was 13000.The enzyme loses its activity completely after being incubated at 70℃ for 90 min- utes.The λ max of the enzyme solution is in 204nm.There are four isoenzymes in B.alboglabra Baileg. |
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| Keywords: | B alboglabra Bail(?)g Superoxide Dismutase Purification |
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