女贞子中总黄酮的超声提取工艺研究

目的:研究女贞子中总黄酮的超声提取工艺.方法:以芦丁作为对照品,总黄酮提取率为指标,采用正交试验优化乙醇浓度、提取次数、超声时间、料液比对总黄酮提取率的影响.结果:所考察的因素对女贞子总黄酮的提取率影响顺序为:提取次数超声时间料液比乙醇浓度,优化的提取条件:乙醇体积分数为40%、料液质量体积比1:25(g:mL),超声提取3次,每次超声处理1 h.结论:该超声提取工艺易于操作,不需加热,提取效率高,提取率为60.6mg·g-1,即每1g女贞子可提取60.6 mg总黄酮.

Study on Ultrasonic Extraction Techniques of Total Flavonoids from Fructus Ligustri

To study ultrasonic extraction techniques of total flavonoids in Fructus ligustri. Methods：Taking Rutin as a reference and extraction ratio of total flavonoids as a index, to study how ethanol concentration, ultrasonic time, solid-liquid ratio and extraction times affect extraction ratio of total flavonoids in Fructus ligustri, by orthogonal design. Results：The results showed that the order of factors to affect total fiavonoid extraction was extraction times 〉 ultrasonic time 〉 solid-liquid ratio 〉 ethanol concentration. The optimum ultrasonic extractions were：A3B1C3D2. ethanol concentration was 40%,ultrasonic time was 60 min,solid-liquid ratio was 1：25 and extraction times was 3. Conclusion：The developed techniques are easy to operate, improve extraction rates and need no heating.