响应面分析法优化金线莲多糖提取过程的研究

以经乙醇提取黄酮后的金线莲滤渣为材料,利用响应面法优化多糖的提取过程.在单因素实验的基础上,分别选定液料比、提取时间和温度3个水平进行响应面实验,建立金线莲多糖提取率的二次回归方程,通过回归分析及岭脊分析得到优化组合条件.研究结果表明,当提取过程中液料比为35∶1,提取时间和温度分别为2.2h和83℃时,金线莲多糖提取率的理论预测值为3.42%,验证值为3.47%±0.47%,两者相差不大,提取条件参数可靠.

Optimization of the Extraction Technology of Polysaccharides from Anoectochilus Roxburghii by Response Surface Analysis

The extraction of polysaccharides from the residue of Anoectochilus roxburghii after extraction of flavonoids was studied. According to the single factor-experiments, three levels of the factors （extraction time, temperature and the ratio of water to residue） were selected for the box-behnken factorial design to establish a quadric regression equation for dcscribing the yield of polysaccharides. By response surface analysis, the optimum extraction conditions were obtained as follows： extraction temperature of 83 ℃, time of 2.2 h and water-pueraria residue ratio of 35 ： 1, under which the highest yield of polysaccharides reached with estimated value and verified value being of 3.42 % and 3.47 % ± 0.47 %, respectively.