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| 食管癌相关基因2(ECRG2)包涵体的溶解与纯化 | |
| 引用本文： | 黄革,徐小虎,崔永萍,陆士新.食管癌相关基因2(ECRG2)包涵体的溶解与纯化[J].汕头大学学报(自然科学版),2006,21(2):29-32,48. |
| 作者姓名： | 黄革 徐小虎 崔永萍 陆士新 |
| 作者单位： | 1. 汕头大学医学院法医实验室,广东,汕头,515031;中国协和医科大学肿瘤研究所,北京,100021 2. 汕头大学医学院法医实验室,广东,汕头,515031 3. 中国协和医科大学肿瘤研究所,北京,100021 |
| 基金项目： | 国家研究发展基金;汕头大学校科研和教改项目 |
| 摘 要： | 采用生物工程的手段,用插有食管癌相关基因2(ECRG2)开放阅读框(ORF)的原核表达质粒pEGX-4T-1转化大肠杆菌E.coli,诱导表达了重组的GST-ECRG2融合蛋白;收集GST-ECRG2融合蛋白包涵体,经SKL溶解后在PBS中透析复性,用GST亲和层析柱纯化,从而获得一定数量的高纯度重组ECRG2蛋白. |
| 关 键 词： | ECRG2 包涵体 纯化 |
| 文章编号： | 1001-4217（2006）02-0029-04 |
| 收稿时间： | 2005-12-19 |
| 修稿时间： | 2005-12-19 |
Solution and Purification of ECRG2 Recombinant Fusion Protein Inclusion Body |
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| HUANG Ge,XU Xiao-hu,CUI Yong-ping,LU Shi-xin.Solution and Purification of ECRG2 Recombinant Fusion Protein Inclusion Body[J].Journal of Shantou University(Natural Science Edition),2006,21(2):29-32,48. | |
| Authors: | HUANG Ge XU Xiao-hu CUI Yong-ping LU Shi-xin |
| Institution: | 1.Medical School, Shantou University, Shantou 515031, Guangdong, China; 2. Cancer Institute, Peking Union Medical College. Beijing 100021, China |
| Abstract: | In this study, ORF of ECRG2 cDNA was cloned into the pEGX-4T-1 vector and expressed in Eseheriehia eoli as a GST fusion protein. Inclusion body GST-ECRG2 was collected, dissolved in SKL, refolded in PBS and purified using GST affinity column. After optimization,purified protein has been obtained at high yield. |
| Keywords: | ECRG2 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |