| 超氧化物歧化酶修饰产物结构和性能研究 |
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| 引用本文： | 韩薇妍,龚平,田平芳.超氧化物歧化酶修饰产物结构和性能研究[J].北京化工大学学报(自然科学版),2010,37(2):117-120. |
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| 作者姓名： | 韩薇妍 龚平 田平芳 |
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| 作者单位： | 1. 北京联合大学 生物化学工程学院，北京 100023； 2. 北京化工大学 生命科学与技术学院，北京 100029 |
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| 基金项目： | 北京市教委科技发展计划项目 |
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| 摘 要： | 以β-环糊精的链状衍生物和环状衍生物分别修饰超氧化物歧化酶(SOD),生成SOD的两种修饰产物。采用化学分析方法测定衍生物的醛基、修饰产物的自由氨基以及SOD的活性,并利用红外光谱表征衍生物和修饰产物的结构。对修饰产物的性能研究发现,超氧化物歧化酶经β-环糊精环状衍生物修饰后耐酸性提高了3.6倍,热稳定性也有所提高,而链状衍生物修饰的SOD热稳定性降低了37%。红外光谱分析显示,两种修饰产物的红外谱图中均带有相应的环糊精衍生物的特征吸收带,表明β-环糊精对SOD的修饰是成功的。
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| 关 键 词： | 超氧化物歧化酶 β-环糊精衍生物 修饰 红外光谱 |
Structure and properties of modified superoxide dismutase |
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| HAN WeiYan,GONG Ping,TIAN PingFang.Structure and properties of modified superoxide dismutase[J].Journal of Beijing University of Chemical Technology,2010,37(2):117-120. |
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| Authors: | HAN WeiYan GONG Ping TIAN PingFang |
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| Institution: | 1. Biological and Chemical College of Beijing Union University, Beijing 100023; 2. College of Life Science and Technology， Beijing University of Chemical Technology, Beijing 100029, China |
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| Abstract: | Superoxide dismutase(SOD)was separately modified by chain and ring derivatives of β-cyclodextrin,resulting in two kinds of products,chain SOD(CSOD)and ring SOD(RSOD),respectively.The contents of al-dehyde and free amino groups,and SOD activities were determined by means of chemical analysis.The typical ab-sorption bands of β-cyclodextrin derivatives were observed in the FTIR spectra of RSOD and CSOD,demonstrating that SOD had been successfully modified by the β-cyclodextrin.The acid-resistance of RSOD was found to be 3.6 times greater than that of SOD and the thermal stability was markedly increased.In contrast,the thermal stability of CSOD was decreased by 37% relative to that of SOD. |
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| Keywords: | superoxide dismutase modification FT-IR spetrum |
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