羧基聚苯乙烯微球共价连接蛋白的定量分析

通过微球表面的羧基在有催化剂的条件下与蛋白质氨基共价连接，并且对微球上包被的蛋白质含量进行测定，探讨了初始加入蛋白量和混匀反应时间对微球蛋白包被率的影响。结果表明羧基微球包被上的蛋白可以精确定量；在蛋白初始加入量为400~1200μg范围内，初始加入蛋白量越多则微球包被蛋白量会增加；确定了混匀反应时间为1h是微球包被蛋白实验的最佳选择。

The quantitative analysis of covalently linked protein for carboxyl polystyrene microspheres

Protein amino groups have been covalently attached to the surface of polystyrene microspheres bearing carboxyl groups in the presence of a catalyst. By determining the amount of protein on the surface of the microspheres, the effects of varying the amount of initially added protein and the blending time on the protein coating ratio has been explored. The results show that the amount of protein on the surface of the microspheres can be accurately quantified, and the amount of protein coated on the surface of the microspheres initially increases as more protein is added, with initially added protein in the range of 400-1200μg. The experiments also showed that the ideal blending time is one hour.