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表达胰岛素的毕赤酵母生长动力学及诱导策略
引用本文:董鹏,陈劲春,冷雪.表达胰岛素的毕赤酵母生长动力学及诱导策略[J].北京化工大学学报(自然科学版),2006,33(3):37-41.
作者姓名:董鹏  陈劲春  冷雪
作者单位:北京化工大学,生命科学与技术学院,北京,100029
摘    要:对本实验室构建的一株重组巴斯德毕赤酵母基因工程菌的生长及诱导进行了研究,结果表明生长阶段菌体的生长与限制性基质甘油残留浓度的关系符合Monod关系式,细胞最大生长比速率为0.204h-1,饱和常数为24.3g/L。经参数推导得理论最大细胞对甘油得率为0.497g/g,以及菌体生长维持系数为0.007g/g·h,后者说明此株工程菌有高密度发酵的潜力。诱导阶段补加酵母提取物、大豆蛋白胨和微量元素能有效提高目的蛋白产量。在发酵罐上罐试验中,目的蛋白产量达256mg/L,蛋白产量是摇瓶发酵蛋白产量的5倍多。

关 键 词:巴斯德毕赤酵母  生长动力学  诱导策略  胰岛素  巴斯德毕赤酵母  生长动力学  诱导策略  胰岛素
收稿时间:2005-09-09
修稿时间:2005年9月9日

Studies of the growth kinetics and induction strategy with a strain of recombinant yeast Pichia Pastoris expressing the human insulin gene
DONG Peng,CHEN Jin-chun,LENG Xue.Studies of the growth kinetics and induction strategy with a strain of recombinant yeast Pichia Pastoris expressing the human insulin gene[J].Journal of Beijing University of Chemical Technology,2006,33(3):37-41.
Authors:DONG Peng  CHEN Jin-chun  LENG Xue
Institution:College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029
Abstract:Growth kinetics and induction strategy of a genetically engineered yeast Pichia Pastoris which boring human insulin gene were studied in this paper. The results indicated that the relationship of specific growth rate(μ) and residual concentration of glycerol is accorded with Monod Equation in phase of cell growth in which μmax=0.204h-1, and Ks=24.3g/L. The maximum cell yield of glycerol Y*X/S=0.497 g/g, and growth maintaining coefficient m=0.007(g/g·h), latter evidenced that the strain has potential of high density cultivation. It was discovered that the heterologous protein concentration could be increased efficiently by batch-fed yeast extract, peptone and trace elements in the phase of induction. The results that heterologous protein concentration reached 256mg/L in 10L fermentor fermentation, which is 4 times more than flask fermentation level, may guide industrial production.
Keywords:Pichia Pastoris  growth kinetics  induction strategy  insulin
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