水产品中麻痹性贝类毒素的微流体免疫检测技术研究

鉴于我国在贝类毒素检测方面相对薄弱的现状，将微流体免疫芯片技术和化学发光分析法相结合来检测贝类毒素，旨在探索一种快速、高效、低耗以及灵敏的检测手段以弥补方法缺陷。选择研究最为广泛的麻痹性贝类毒素-石房蛤毒素（STX）作为研究对象，利用基于酶联免疫反应（ELISA）为原理的石房蛤毒素检测试剂盒在微流体免疫芯片-化学发光检测平台进行实验，绘制出STX标准曲线并计算出检测限。首先在羧基聚苯乙烯微球表面包被抗体并进行定性分析，然后对底物流速和底物溶液的稀释倍数等进行优化，最后对市场上的贝类进行了实际样品的检测。通过绘制标准曲线得到STX的检测限为0.005675ng/mL，而传统方法的检测限为5ng/mL，表明应用微流体芯片技术可以大幅度提高食品安全检测的灵敏度。

Detection of paralytic shellfish toxins by microfluidic immunoassay

In view of the relatively poor ability to detect shellfish toxins in China, shellfish poisoning has become a very serious food safety problem. In this article, we show how microfluidic immune chip technology and chemiluminescence analysis can be combined to detect shellfish toxins, giving a fast, efficient, low-power and sensitive detection method superior to conventional methods. The most widely studied paralytic shellfish toxin-Saxitoxin (STX) was selected as the research object. Experiments were carried out on a microfluidic chemiluminescence detection platform by using a kit for the detection of STX. Then the standard curve was plotted and the detection limit was calculated. In the early stages of the experiment, the antibodies were coated on carboxyl polystyrene microspheres and analyzed qualitatively. The optimization of flow rate and dilution ratio of the substrate solution was subsequently carried out. Finally, the shellfish in the market were studied. By plotting the standard curve, the detection limit of STX was found to be 0.005675ng/mL, proving that sensitivity in food safety testing can be greatly improved by the use of microfluidic chip technology.