耐高COD淀粉废水的高产油脂粘红酵母的驯化和筛选

为减轻高COD淀粉废水对产油菌株粘红酵母生长的抑制，提高油脂产量，本文利用淀粉废水对该菌株进行了耐高COD梯度驯化，并采用流式细胞仪对经尼罗红染色的粘红酵母细胞进行了高油脂含量菌株的筛选。结果表明：经多次驯化后，粘红酵母耐受淀粉废水COD高达75000mg/L；流式细胞仪筛选得到一株油脂含量为25.7%（质量分数）的粘红酵母，比原始菌株油脂提高了140%。400L发酵罐实验表明，在初始COD 75000mg/L，葡萄糖质量浓度为36g/L，pH4.8及30℃条件下，培养33h后，粘红酵母生物量达25.3g/L，菌体油脂含量为29.5%，COD降至5600mg/L，降解率为92.5%。

Domestication and screening of Rhodotorula glutinis with resistance to high chemical oxygen demand (COD) starch wastewater for lipid production

In order to decrease the inhibition of the high chemical oxygen demand (COD) wastewater on the growth of an oil-producing strain-Rhodotorula glutinisand thereby increase oil production, the domestication of strains with resistance to high COD was performed in starch wastewater. Screening of strains with high lipid production obtained from Nile Red staining of Rhodotorula glutinis cells was completed by flow cytometry. After multiple domestication steps, Rhodotorula glutinis could tolerate starch wastewater with COD up to 75000mg/L. One Rhodotorula glutinis with a lipid content of 25.7% screened by flow cytometry had a lipid content which was 140% higher than the original. A 400L fermentor experiment showed for initial conditions of 75000mg/L COD, 36g/L glucose and pH 4.8, after culturing for 33h at 30℃, the biomass could reach 25.3g/L and the bacterial lipid content reached 29.5%, while COD decreased to 5600mg/L. The degradation ratio was 92.5%.