绿僵菌与小檗碱复配制备水分散粒剂

将金龟子绿僵菌（Metarhizium anisopliae）和小檗碱复配为水分散粒剂，可以起到既抗虫又抗病的双重效果，并且可降低田间施药的成本。测得小檗碱对绿僵菌的有效中浓度（EC₅₀）为47.2 mg/mL，表明小檗碱对绿僵菌没有明显的抑制作用；根据以往绿僵菌的田间试验及小檗碱的抗真菌实验，确定小檗碱的最终添加量为3%（质量分数）。在单因素试验的基础上，利用响应面法确定水分散粒剂的配方为：25%绿僵菌孢子粉、3%小檗碱、3%乳化剂NP-10（润湿剂）、2.1%亚甲基双甲基萘磺酸钠（分散剂）、5.3%木质素磺酸钙（分散剂）、18%海藻酸钠（崩解剂）、1.7%羧甲基纤维素钠（黏结剂），用硅藻土（载体）补足100%（均为各成分占制剂的质量分数）。按照此配方在造粒机中制备了水分散粒剂，质量检测结果表明：所制备的水分散粒剂的崩解时间为174 s，孢子萌发率为85.4%，孢子悬浮率为86.34%，含水量为1.88%，杂菌率为2.01%，符合相关行业标准。

Preparation of water-dispersible granules by combination of Metarhizium anisopliae and berberine

Combining Metarhizium anisopliae and berberine into water-dispersible granules offers the dual effects of insecticidal action and disease control, and can reduce the cost of field application. The median effective concentration (EC₅₀) of berberine against M. anisopliae was 47.2 mg/mL, indicating that berberine has no obvious inhibi-tory effect on M. anisopliae. According to previous field trials of M. anisopliae and antifungal experiments with berberine, the final amount of berberine was determined to be 3% (mass fraction). Based on single factor tests, the response surface method was used to determine the optimized formulation of the water-dispersible granules:25% M. anisopliae spore powder, 3% berberine, 3% emulsifier NP-10 (wetting agent), 2.1% sodium methylene dimethyl naphthalene sulfonate (dispersant), 5.3% calcium lignosulfonate (dispersant), 18% sodium alginate (disintegrant), 1.7% sodium carboxymethylcellulose (binder), and diatomaceous earth (carrier) as the remainder (the amount of ingredients refers to the mass fraction used in the preparation). Using this formulation, water-dispersible granules were prepared in a granulator. Quality test results showed that the disintegration time of the prepared water-dispersible granules was 174 s, the spore germination rate was 85.4%, the spore suspension rate was 86.34%, the water content was 1.88%, and the rate of microbial contaminants was 2.01%, which meets the relevant industry standards.